计算溶液所需的质量、体积或浓度。
BioReagent,生物染色剂,用于显微镜,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
细菌的荚膜具有保护细菌抵抗吞噬和消化的作用,可增加细菌的侵袭力,荚膜不易被普通染色法染色,需经特殊染色法染色后才能着色,易于观察,细菌荚膜的鉴定,对细菌的分型和鉴别有至关重要的作用。
阿拉丁荚膜染色液 (Hiss法) 又称硫酸铜染色液,细菌荚膜被染成蓝紫色,细菌被染成深蓝色。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
产品组分及储存条件:
| C1510424 | Component | 2×50mL | Storage |
| C1510424A | Hiss染色液 | 50mL | RT |
| C1510424B | 硫酸铜溶液 | 50mL | RT |
自备材料:
载玻片、盖玻片、酒精灯、显微镜
操作步骤(仅供参考):
1、细菌涂片,空气自然干燥,乙醇固定。
2、滴加Hiss染色液于玻片上,火焰上加热使有蒸汽冒出为止或者染色5~7min,勿水洗。
3、用硫酸铜溶液冲洗,勿水洗。
4、自然晾干或吸水纸吸干,在低倍镜下寻找有荚膜的细菌,并转高倍镜或油镜下仔细辨认。
染色结果:
| 荚膜 | 淡紫色或蓝色 |
| 菌体 | 菌体紫色 |
注意事项:
1、有效期内已开封荚膜染色液应在6个月内用完,每次用后尽量密封好,以免挥发或变质。
2、存储时间较长后易产生沉淀,如果镜下发现颗粒粗糙者应过滤后使用或弃用。
3、目的样本尽量新鲜。样本和荚膜染色液分布应均匀。
4、样本的纯度和浓度应适宜,浓度过低时在镜下很难寻找到新型隐球菌。
5、为了您的安全和健康,请穿实验服并戴一次性手套操作。
The capsule of bacteria can protect the bacteria against phagocytosis and digestion, and enhance the invasiveness of bacteria. The capsule is not easy to be stained by ordinary staining methods and needs to be dyed by special staining methods to be colored for easy observation. The identification of bacterial capsules plays a crucial role in the typing and differentiation of bacteria.
Aladdin Capsule Stain Solution (Hiss Method), also known as Copper Sulfate Staining Solution, stains bacterial capsules blue-purple and bacterial cells dark blue. This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
| C1510424 | Component | 2×50mL | Storage |
| C1510424A | Hiss Staining Solution | 50mL | RT |
| C1510424B | Copper Sulfate Solution | 50mL | RT |
Self-prepared Materials:
Glass slides, coverslips, alcohol lamps, microscopes
Operating Procedures (For Reference Only):
1. Prepare a bacterial smear, air-dry it naturally, and fix it with ethanol.
2. Add Hiss staining solution onto the slide, heat it over an alcohol lamp until steam emerges, or stain for 5–7 minutes. Do not rinse with water.
3. Rinse the slide with copper sulfate solution. Do not rinse with water.
4. Air-dry it naturally or blot it dry with absorbent paper. Locate capsulated bacteria under a low-power microscope, then switch to a high-power microscope or oil immersion lens for careful identification.
Staining Results:
| Capsule | Pale purple or blue |
| Bacterial cells | Purple |
Precautions:
1. Opened capsule staining solution within its shelf life should be used up within 6 months. Seal the bottle tightly after each use to prevent volatilization or deterioration.
2. Precipitation may occur after long-term storage. If coarse particles are observed under the microscope, filter the solution before use or discard it.
3. The target sample should be as fresh as possible. Both the sample and capsule staining solution must be evenly distributed.
4. The sample should have appropriate purity and concentration. It will be difficult to locate Cryptococcus neoformans under the microscope if the concentration is too low.
5. For your safety and health, wear a lab coat and disposable gloves during the operation.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | C1510424 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Suitable for microbiology 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →