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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
钙（calcium）通常是哺乳动物机体内最为丰富的矿物质，参与调控众多的细胞生命活动过程，也是最为重要的细胞内调控因子之一。钙能够以自由离子或结合钙离子的复合物两种形式存在，例如磷酸钙和碳酸钙复合物即为骨骼组织的组成成分。包括肌肉收缩、细胞粘附、激素/神经递质释放、糖原代谢、细胞增殖/分化、血凝、神经或突触传递以及骨骼结构支持在内的大量生理过程都受到钙信号的调控。细胞特异性钙信号系统完整性的缺陷会导致一些疾病的发生。虽然人体内 99%的钙存在于骨骼和牙齿中，但临床上非常关注血液的钙水平，即血钙。血钙水平主要通过甲状旁腺激素调节骨骼储存钙或者释放钙，以避免血钙过高或者过低。血清中的钙主要以三种形式存在：约 50%的游离钙离子、约 45%的蛋白结合钙、约 5%的钙复合物（主要是柠檬酸钙）。钙的水平通常和磷的水平负相关。钙含量检测试剂盒（邻甲酚酞络合酮, 微量法）提供了一种简单、方便、快速的钙离子含量检测方法，适用于动植物组织、细胞、血清（浆）或其他液体样本。其原理是钙离子与邻甲酚酞络合酮在碱性条件下反应产生紫色复合物，通过检测 575 nm 处的吸光度即可定量检测钙离子含量。
Calcium (calcium) is typically the most abundant mineral in the bodies of mammals and is involved in regulating numerous cellular life processes. It is also one of the most important intracellular regulatory factors. Calcium can exist in two forms: as free ions or as complexes with bound calcium ions, such as calcium phosphate and calcium carbonate, which are components of bone tissue. A wide range of physiological processes, including muscle contraction, cell adhesion, hormone/neurotransmitter release, glycogen metabolism, cell proliferation/differentiation, blood coagulation, neural or synaptic transmission, and bone structure support, are regulated by calcium signaling. Defects in the integrity of cell-specific calcium signaling systems can lead to the development of various diseases. Although 99% of the calcium in the human body is found in bones and teeth, clinical attention is often focuse on blood calcium levels, known as serum calcium. Blood calcium levels are primarily regulated by parathyroid hormone, which controls the storage or release of calcium from bones to prevent hypercalcemia or hypocalcemia. Calcium in serum exists primarily in three forms: approximately 50% as free calcium ions, about 45% as protein-bound calcium, and about 5% as calcium complexes (mainly calcium citrate). Calcium levels are typically inversely related to phosphorus levels. Calcium Content Assay Kit (o-Cresolphthalein Complexone, Micro Method) provides a simple, convenient, and rapid method for detecting calcium ion content, suitable for animal and plant tissues, cells, serum (plasma) or other Liquid samples. The principle is based on the reaction of calcium ions with o-cresolphthalein complexone under alkaline conditions to form a purple complex. The calcium ion content can be quantitatively determined by measuring the absorbance at 575 nm.
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