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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
蛋白质含有三种含硫氨基酸:甲硫氨酸、胱氨酸和Cys。其中,Cys是唯一一种含有巯基的含硫氨基酸,从甲硫氨酸转化而来,并且可与胱氨酸互相转化。Cys参与蛋白质二硫键的形成,经常是蛋白质活性中心的组成部分,还可以为其它生理生化反应提供巯基。此外,Cys大量积聚在皮肤和粘膜表面,在角蛋白生成中维持重要的巯基酶的活性,并且补充巯基,以维持皮肤的正常代谢,调节表皮最下层的色素细胞生成的底层黑色素。具有美白、解毒、改善炎症和过敏性皮肤等作用。 测定原理:Cys还原磷钨酸生成钨蓝,在600nm处有吸收峰;通过600nm吸光度,计算Cys含量。
There are three sulfur-containing amino acids in proteins: methionine, cystine, and cysteine (Cys). Among them, Cys is the only sulfur-containing amino acid that contains a sulfhydryl (-SH) group. It is derived from methionine and can be interconverted with cystine. Cys participates in the formation of protein disulfide bonds, often constitutes part of the active site of proteins, and can provide sulfhydryl groups for other physiological and biochemical reactions. Furthermore, Cys accumulates abundantly on the surface of the skin and mucous membranes. It maintains the activity of crucial sulfhydryl enzymes involved in keratin production, replenishes sulfhydryl groups to support normal skin metabolism, and regulates the production of underlying melanin by pigment cells in the deepest layer of the epidermis. These functions contribute to its roles in skin whitening, detoxification, and improving inflammatory and allergic skin conditions. Assay Principle: Cys reduces phosphotungstic acid to produce tungsten blue, which exhibits an absorption peak at 600 nm. The Cys content is calculated based on the absorbance at 600 nm.