BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血浆总胆固醇包括 2/3 的胆固醇酯和 1/3 的游离胆固醇，是血脂的重要组分，二者与心脑血管疾病以及多种病理状态密切相关。本试剂盒采用世界卫生组织(WHO)、美国 FDA、中国《全国临床检验操作规程》推荐的总胆固醇和游离胆固醇检测方法，灵敏度高，检测范围 20~5000 µmol/L。可靠性和重复性俱佳。 本试剂盒测定方法采用 CHOD-PAP 终点方法结合经典 GPO Trinder 酶学反应，其原理为：(1) 胆固醇酯酶分解胆固醇酯为游离胆固醇。(2) 胆固醇氧化酶将游离胆固醇氧化，产生过氧化氢。(3) 在过氧化物酶催化下生色底物转化为苯醌亚胺，光密度值与胆固醇浓度成正比。(4)分别计算出总胆固醇浓度与游离胆固醇浓度，二者差值即为胆固醇酯浓度。
Plasma total cholesterol consists of two-thirds cholesterol esters and one-third free cholesterol. It is a key component of blood lipids, and both forms are closely associated with cardiovascular and cerebrovascular diseases as well as various pathological conditions. This kit employs the total cholesterol and free cholesterol detection methods recommended by the World Health Organization (WHO), the U.S. Food and Drug Administration (FDA), and China's National Clinical Laboratory Operating Procedures, offering high sensitivity with a detection range of 20–5000 µmol/L, as well as excellent reliability and reproducibility. The kit utilizes the CHOD-PAP endpoint method combined with the classic GPO Trinder enzymatic reaction. The principle is as follows: (1) Cholesterol esterase hydrolyzes cholesterol esters into free cholesterol; (2) Cholesterol oxidase oxidizes free cholesterol to produce hydrogen peroxide; (3) Under the catalysis of peroxidase, the chromogenic substrate is converted into benzoquinone imine, with the optical density being proportional to the cholesterol concentration; (4) The total cholesterol concentration and free cholesterol concentration are calculated separately, and the difference between the two values represents the cholesterol ester concentration.
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