BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
在实体组织和细胞内，胆固醇酯既是构成细胞质膜的成分，也是细胞内脂滴的主要组分。细胞内胆固醇过度聚集与动脉粥样硬化泡沫细胞形成密切相关。实体组织和细胞的胆固醇测定远比血液胆固醇测定复杂。本试剂盒避免了有毒的有机溶剂抽提、繁杂的氮气吹干和脂质复溶等步骤，采用高效能试剂裂解细胞和提取胆固醇，优化了酶学反应和操作步骤，简单易行，灵敏度高，线性范围20~5000 µmol/L。 本试剂盒采用 WHO、中国《全国临床检验操作规程》推荐的酶学方法，结合经典GPOTrinder 酶学反应，原理如下：(1) 胆固醇酯酶分解胆固醇酯为游离胆固醇。(2) 胆固醇氧化酶将游离胆固醇氧化，反应过程产生过氧化氢。(3) 在过氧化物酶催化下进行生色反应，在 550nm 有最大吸收峰，光密度值与胆固醇浓度成正比。(4)分别计算出总胆固醇浓度与游离胆固醇浓度，二者差值即为胆固醇酯浓度。
In solid tissues and cells, cholesterol esters are not only components of the cell plasma membrane but also major constituents of intracellular lipid droplets. Excessive intracellular cholesterol accumulation is closely associated with the formation of atherosclerotic foam cells. Cholesterol determination in solid tissues and cells is far more complex than that in blood. This kit eliminates the need for toxic organic solvent extraction, cumbersome nitrogen drying, and lipid reconstitution steps. It employs high‑efficiency reagents for cell lysis and cholesterol extraction, with optimized enzymatic reactions and procedures, making it simple and easy to use while offering high sensitivity and a linear range of 20–5000 µmol/L. This kit utilizes the enzymatic method recommended by the WHO and China’s National Clinical Laboratory Operating Procedures, combined with the classic GPO Trinder enzymatic reaction. The principle is as follows: (1) Cholesterol esterase hydrolyzes cholesterol esters into free cholesterol; (2) Cholesterol oxidase oxidizes free cholesterol, producing hydrogen peroxide during the reaction; (3) A chromogenic reaction is catalyzed by peroxidase, producing a product with a maximum absorption peak at 550 nm, where the optical density is proportional to the cholesterol concentration; (4) The total cholesterol concentration and free cholesterol concentration are calculated separately, and the difference between the two values represents the cholesterol ester concentration.
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