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CAD是木质素生物合成途径中的关键酶之一,是木质素单体合成反应中的最后一步,催化多种不同的肉桂醛(香豆醛、芥子醛以及松柏醛等)生成与之相应的肉桂醇。该酶多存在于高等植物、酵母、菌类中,研究该酶可以探讨多种生物细胞发育过程中木质素沉积的代谢机理,为减少水果石细胞含量提高其品质提供依据。 测定原理:CAD催化肉桂醇和NADP⁺生成肉桂醛和NADPH,在340nm下测定NADPH生成速率,即可反映CAD活性。
CAD is one of the key enzymes in the lignin biosynthesis pathway, catalyzing the final step in the synthesis of monolignols. It converts various cinnamaldehydes (such as p-coumaraldehyde, sinapaldehyde, and coniferaldehyde) into their corresponding cinnamyl alcohols. This enzyme is primarily found in higher plants, yeast, and fungi. Studying CAD helps elucidate the metabolic mechanisms of lignin deposition during cell development in various organisms, providing a basis for reducing stone cell content in fruits and improving their quality. Determination Principle: CAD catalyzes the conversion of cinnamyl alcohol and NADP⁺ to cinnamaldehyde and NADPH. CAD activity is determined by measuring the rate of NADPH production at 340 nm.