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辅酶Q（Coenzyme Q，CoQ）是一类广泛存在于生物体内的脂溶性醌类化合物，亦称泛醌。它在呼吸链中介导质子移位与电子传递，作为关键的递氢体，是细胞呼吸和代谢的激活剂，同时具备抗氧化和非特异性免疫增强的功能，并可激活多种酶。不同物种来源的辅酶Q其侧链异戊烯单位数目存在差异：人类及其他哺乳动物含有10个异戊烯单位，因此称为辅酶Q10。辅酶Q10是辅酶Q家族中的重要成员，定位于线粒体内膜，广泛参与体内的生物代谢过程。 辅酶Q10不仅能够为心脏提供能量支持，还具备突出的抗氧化作用，可清除自由基，抑制血管壁脂质过氧化，预防动脉粥样硬化，且无毒副作用。其具体作用主要体现在以下四个方面： ① 保护心脏：辅酶Q10有助于增加心肌供氧，预防突发性心脏病，在心肌缺氧状态下发挥关键改善作用。 ② 护肤抗衰：长期使用辅酶Q10能有效延缓皮肤老化，减少面部皱纹。 ③ 抗疲劳：辅酶Q10维持细胞的良好状态，使机体充满活力、精力充沛、脑力增强。作为细胞自身生成的天然抗氧化剂和代谢启动剂，它具有保护和恢复生物膜结构完整性、稳定膜电位的作用，同时增强机体非特异性免疫功能，从而表现出显著的抗疲劳效果。 ④ 辅助防癌：研究表明，辅酶Q10具有一定的抗肿瘤作用，临床上对晚期转移性癌症患者显示出一定疗效。 本试剂盒的检测原理基于：在碱性条件下，待测样品中的辅酶Q10与EC试剂反应，取代其甲氧基生成蓝色化合物；通过分光光度计测定620 nm波长处的吸光度值，结合标准曲线可定量测定辅酶Q10含量。本产品适用于花生、牛肉、沙丁鱼、保健品等食品中辅酶Q10的检测，仅供科研使用，不适用于临床诊断或其他用途。
Coenzyme Q (CoQ) is a class of lipid-soluble quinone compounds widely present in living organisms, also known as ubiquinone. It mediates proton translocation and electron transfer in the respiratory chain, serving as a key hydrogen carrier. It is an activator of cellular respiration and metabolism, while also possessing antioxidant and non-specific immune-enhancing functions, and can activate various enzymes. The number of isoprene units in the side chain of Coenzyme Q varies depending on the biological source: humans and other mammals contain 10 isoprene units, hence it is referred to as Coenzyme Q10. Coenzyme Q10 is an important member of the Coenzyme Q family, localized in the inner mitochondrial membrane, and widely participates in the body's metabolic processes. Coenzyme Q10 not only provides energy support for the heart but also exhibits outstanding antioxidant effects. It can scavenge free radicals, inhibit vascular wall lipid peroxidation, and prevent atherosclerosis, all without toxic side effects. Its specific effects are mainly reflected in the following four aspects: ① Heart Protection: Coenzyme Q10 helps increase myocardial oxygen supply, prevents sudden heart disease, and plays a key role in improving conditions during myocardial hypoxia. ② Skin Care and Anti-aging: Long-term use of Coenzyme Q10 can effectively delay skin aging and reduce facial wrinkles. ③ Anti-fatigue: Coenzyme Q10 maintains cells in good condition, keeping the body energetic, vigorous, and enhancing brain function. As a natural antioxidant and metabolic activator produced by cells themselves, it protects and restores the structural integrity of biofilms, stabilizes membrane potential, and enhances the body's non-specific immune function, thereby demonstrating significant anti-fatigue effects. ④ Adjuvant Anti-cancer: Research indicates that Coenzyme Q10 possesses certain anti-tumor effects and has shown some efficacy in clinical settings for patients with advanced metastatic cancer. The detection principle of this kit is based on the following: Under alkaline conditions, the Coenzyme Q10 in the test sample reacts with the EC reagent, replacing its methoxy group to form a blue compound. The absorbance value at 620 nm is measured using a spectrophotometer, and the Coenzyme Q10 content can be quantitatively determined by referencing a standard curve. This product is suitable for detecting Coenzyme Q10 in foods such as peanuts, beef, sardines, and health products. It is intended for research use only and is not suitable for clinical diagnosis or other purposes.
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