BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
4-香豆酸辅酶A连接酶是催化桂皮酸形成咖啡酸、香豆酸的酶。该酶多存在于高等植物、酵母、菌类可溶性部分物质，属于细胞木质素合成途径中间的关键酶，研究该酶可以探讨多种生物细胞发育过程中木质素沉积的代谢机理，为减少水果石细胞含量提高其品质提供依据。 该试剂盒检测原理是以香豆酸作为底物，在酶促反应的最适条件下采用每隔一定时间测定产物生成量的方法，于分光光度计333nm处检测吸光度，以吸光度变化所需酶量进行计算，主要用于植物组织的裂解液或匀浆液、血清等样品中内源性的4-香豆酸辅酶A连接酶活性，尤其适用于检测水果中4-香豆酸辅酶A连接酶活性。本产品仅用于科研领域，不适用于临床诊断或其他用途。
4-Coumarate-CoA ligase is an enzyme that catalyzes the formation of caffeic acid and coumaric acid from cinnamic acid. This enzyme is predominantly found in the soluble fractions of higher plants, yeast, and fungi, and is a key enzyme in the cellular lignin biosynthesis pathway. Studying this enzyme allows for the exploration of the metabolic mechanisms underlying lignin deposition during the developmental processes of various biological cells, providing a basis for reducing the stone cell content in fruits and improving their quality. The detection principle of this kit is as follows: Using coumaric acid as a substrate, under the optimal conditions for the enzymatic reaction, the amount of product generated is measured at fixed time intervals. The absorbance is detected at 333 nm using a spectrophotometer, and the enzyme activity is calculated based on the amount of enzyme required for the change in absorbance. This kit is primarily used for detecting endogenous 4-coumarate-CoA ligase activity in samples such as plant tissue lysates or homogenates, and serum. It is particularly suitable for measuring 4-coumarate-CoA ligase activity in fruits. This product is intended for research use only and is not suitable for clinical diagnostic or other purposes.
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