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肉桂酸-4-羟基化酶是催化桂皮酸形成咖啡酸、香豆酸的酶，该酶多存在于高等植物、酵母、菌类可溶性部分物质，属于细胞木质素合成途径中间的关键酶，研究该酶可以探讨多种生物细胞发育过程中木质素沉积的代谢机理，为减少水果石细胞含量、提高其品质提供依据。 该试剂盒的检测原理是以肉桂酸作为底物，在酶促反应的最适条件下采用每隔一定时间测定产物生成量的方法，于分光光度计或酶标仪290nm处检测吸光度，以吸光度变化所需酶量进行计算。主要用于植物组织的裂解液或匀浆液、血清等样品中内源性的肉桂酸-4-羟基化酶活性，尤其适用于检测水果中肉桂酸-4-羟基化酶活性。本产品仅用于科研领域，不适用于临床诊断或其他用途。
Cinnamate-4-hydroxylase is an enzyme that catalyzes the conversion of cinnamic acid into caffeic acid and coumaric acid. This enzyme is predominantly found in the soluble fractions of higher plants, yeasts, and fungi. It serves as a key intermediate enzyme in the lignin biosynthesis pathway of plant cells. Studying this enzyme allows for the exploration of the metabolic mechanisms underlying lignin deposition during the developmental processes of various biological cells, providing a basis for reducing the stone cell content in fruits and improving their quality. The detection principle of this kit is based on using cinnamic acid as a substrate. Under optimal conditions for the enzymatic reaction, the method of measuring the amount of product generated at specific time intervals is employed. The absorbance is detected at 290 nm using a spectrophotometer or a microplate reader, and the enzyme activity is calculated based on the amount of enzyme required for the change in absorbance. This kit is primarily intended for measuring endogenous cinnamate-4-hydroxylase activity in samples such as plant tissue lysates or homogenates, serum, etc. It is particularly suitable for detecting cinnamate-4-hydroxylase activity in fruits. This product is intended for research use only and is not for use in diagnostic procedures or other purposes.
危险品化学品经营许可证（带存储）