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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
细胞分裂素氧化酶(CKO/CKX,EC 1.5.99.12)既能特异性催化细胞分裂素类异戊二烯侧链的不饱和键,又能控制CK的合成与降解以稳定植物体内CK的含量,是目前发现的唯一可促进内源CK降解的关键酶。 细胞分裂素氧化酶(CKO/CKX)催化底物进一步还原2,6-二氯酚靛酚(DCPIP),使该物质在600nm处的吸光值减小,通过检测600nm处的下降速率进而得到CKO/CKX酶活性大小。
Cytokinin oxidase (CKO/CKX, EC 1.5.99.12) specifically catalyzes the unsaturated bond on the isoprenoid side chain of cytokinins, and also controls cytokinin synthesis and degradation to maintain stable cytokinin levels in plants. It is currently the only key enzyme known to promote the degradation of endogenous cytokinins. Cytokinin oxidase (CKO/CKX) catalyzes the reduction of the substrate 2,6-dichlorophenolindophenol (DCPIP), leading to a decrease in absorbance at 600 nm. The enzyme activity of CKO/CKX is determined by measuring the rate of decrease in absorbance at this wavelength.