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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
叶绿体中所含色素主要有两大类,叶绿素(包括叶绿素 a 和叶绿素 b)和类胡萝卜素(包括胡萝卜素和叶黄素),它们与类囊体膜上的蛋白质结合,成为色素蛋白复合体,其含量多少及其组成决定了植物对不同光的吸收、利用效率,常常作为研究光合生理的重要指标。 根据叶绿体色素提取液对可见光谱的吸收,在649nm和665nm处测定叶绿素提取物的吸光值,在470nm处测定类胡萝卜素;然后利用经验公式计算出样品中叶绿素a含量、叶绿素b含量、叶绿素总含量及类胡萝卜素含量。
The pigments in chloroplasts mainly consist of two categories: chlorophylls (including chlorophyll a and chlorophyll b) and carotenoids (including carotene and xanthophylls). These pigments bind to proteins on the thylakoid membrane to form pigment-protein complexes. Their content and composition determine the efficiency of light absorption and utilization by plants, and they are often used as important indicators in photosynthetic physiology research. Based on the absorption spectrum of the chloroplast pigment extract, the absorbance of the chlorophyll extract is measured at 649 nm and 665 nm, and that of carotenoids is measured at 470 nm. Empirical formulas are then used to calculate the chlorophyll a content, chlorophyll b content, total chlorophyll content, and carotenoid content in the sample.