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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
叶绿体复合体Ⅴ又称F1F0-ATP合酶,由F1和F0两个亚单位组成。该酶利用呼吸链产生的质子电化学梯度催化ATP合成,也可逆过程水解ATP。复合体Ⅴ是线粒体氧化磷酸化和叶绿体光合磷酸化合成ATP的关键酶。 测定原理:复合体Ⅴ水解ATP产生ADP和Pi,通过测定Pi增加速率来测定复合体Ⅴ活性。
Chloroplast complex V, also known as F1F0-ATP synthase, is composed of two subunits, F1 and F0. This enzyme utilizes the proton electrochemical gradient generated by the respiratory chain to catalyze ATP synthesis and can also hydrolyze ATP in the reverse reaction. Complex V is a key enzyme in ATP synthesis through oxidative phosphorylation in mitochondria and photophosphorylation in chloroplasts. Determination Principle: Complex V hydrolyzes ATP to produce ADP and Pi. The activity of complex V is determined by measuring the rate of Pi increase.