计算溶液所需的质量、体积或浓度。
EnzymoPure™,生物活性,重组 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
CMP-唾液酸合成酶 ( CMP–sialic acid synthetase, NmCSS),E.C 2.7.7.43,来源于 Neisseria meningitidis.该酶用于活化唾液酸形成 CMP-唾液酸,作为糖基转移酶转移唾液酸的供体,可以作唾液酸糖转移的工具酶。本产品利用大肠杆菌重组表达,纯度高,兼容性好,可以用于一锅多酶体系
反应式
Neu5Ac + CTP —— CMP-Neu5Ac + PPi
使用方法
1.按照反应体系加入底物(Neu5Ac,CTP)以及 5mM MgCl2,可适当加入缓冲盐(如:Tris-HCl),调节 pH 为7.5-8.5,加入适量 NmCSS(根据反应需求);此反应中需加入无机焦磷酸酶可以提高转化率。
2.混匀后,将样品置于 37℃孵育 30-60min。
【注】体系放大时,孵育时间或酶量需要根据实际情况调整,可低速震荡混匀。
【注】多数常见的生物缓冲液适用,比如 Tris 或 PBS,不在此 pH 范围的缓冲液(如醋酸缓冲液)可能适用,但是孵育时间或酶量需要根据实际情况进行优化
注意事项
为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅作科研用途!
CMP–sialic acid synthetase (NmCSS), EC 2.7.7.43, derived from Neisseria meningitidis. This enzyme activates sialic acid to produce CMP-sialic acid, which serves as the sialic acid donor for glycosyltransferases, and acts as a tool enzyme for sialylation transfer reactions. This product is recombinantly expressed in Escherichia coli, featuring high purity and excellent compatibility, and is applicable in one-pot multi-enzyme systems.
Reaction Equation:
Neu5Ac + CTP → CMP-Neu5Ac + PPi
Instructions for Use
Add substrates (Neu5Ac and CTP) and 5 mM MgCl₂ to the reaction system; appropriate buffer salts (e.g., Tris-HCl) may be supplemented to adjust the pH to 7.5–8.5, followed by the appropriate amount of NmCSS according to reaction requirements. Addition of inorganic pyrophosphatase to the system can improve conversion efficiency.
Mix thoroughly and incubate the sample at 37 °C for 30–60 min.
Notes
When scaling up the reaction system, incubation time or enzyme dosage shall be adjusted according to practical conditions; low-speed shaking mixing is recommended.
Most common biological buffers such as Tris and PBS are compatible. Buffers outside this pH range (e.g., acetate buffer) may also be usable, but incubation time and enzyme dosage need to be optimized on a case-by-case basis.
Precautions
For your safety and health, please wear a lab coat and disposable gloves during handling. This product is for research use only!
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | C1524613 | |
| 分析证书 | C1524613 |
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查看 Bioactive 级别指南 → 查看 Recombinant 级别指南 → 查看 EnzymoPure™ 级别指南 →