是否无菌:是
念珠菌清除剂 (500×) 是新一代念珠菌清除剂,对常见真菌污染(如:霉菌,酵母等)也有一定的抑制作用,主要用于清除细胞、血清、培养基中的念珠菌属污染。新一代抗真菌配方,只需2-3天就可以抑制念珠菌增长,一周左右即可清除念珠菌污染。
作用原理:念珠菌清除剂主要是通过特异性的破坏真菌细胞壁的结构,让真菌细胞失去固有形态及完整性,从而影响念珠菌属的正常代谢、离子交换和渗透压,让细胞彻底摆脱念珠菌的困扰,确保细胞的健康生长。
使用说明:
1. 请在收到货后,将试剂管瞬时离心后保存;
2. 用PBS冲洗细胞3-5次,每次都从一个方向冲洗倒掉;
3. 推荐将念珠菌清除剂与完全培养液按1:500比例稀释,加入细胞正常培养;
4. 按照步骤3处理细胞3-6次。
注意事项:
1. 分装储存,务必现配现用;
2. 因本品与抗生素有拮抗作用,因此不得与抗生素混用;
3. 若细胞污染非常严重,建议以1:200的比例使用,污染得到有效控制后降低药物浓度1:500-800继续处理2-3次;
4. 因本产品属于多肽类产品,若部分细胞对本产品敏感,建议使用本品处理2天,然后停药处理1-2天,重复此步骤处理3-5个周期即可;
5. 本试剂仅供研究使用。
Is it sterile: Yes
Candida Clearing Agent (500×) is a next‑generation reagent against candida contamination. It also delivers inhibitory effects on common fungal contaminants including moulds and yeasts. It is mainly adopted to eradicate Candida‑related contamination in cultured cells, serum and culture media. Formulated with innovative anti‑fungal ingredients, it suppresses candida proliferation within 2‑3 days and eliminates candida contamination in roughly one week.
Mechanism of Action: This eliminator specifically disrupts the structure of fungal cell walls. It destroys the inherent shape and structural integrity of fungal cells, and then disturbs normal metabolism, ion exchange and osmotic pressure of Candida species. Consequently, cultured cells get rid of candida contamination and maintain healthy growth.
Instructions for Use:
1. Perform brief centrifugation of the reagent vial upon receipt before storage.
2. Rinse the cell monolayer with PBS 3‑5 times; pour off the rinse solution in a consistent direction every time.
3. Dilute Candida Eliminator with complete culture medium at the recommended dilution ratio of 1:500, then incubate cells under routine culture conditions.
4. Treat cells as described in Step 3 for 3‑6 cycles.
Precautions:
1. Store the reagent in aliquot portions and prepare the working‑solution right before use.
2. Do not mix this product with antibiotics, as antagonistic effects exist between the reagent and antibiotics.
3. In the event of severe fungal contamination, apply the reagent at a 1:200 dilution. Once contamination is well‑controlled, adjust the dilution to 1:500‑800 and carry out another 2‑3 treatments.
4. Since this is a polypeptide‑based product, certain cell lines may exhibit sensitivity. If so, treat cells with the reagent for two days, then continue culturing in reagent‑free medium for 1‑2 days. Repeat the cycle for 3‑5 rounds.
5. This reagent is for research‑only use.