BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
胆碱酯酶（cholinesterase，ChE）属于特异性酯酶，可分为两大类： ① 乙酰胆碱酯酶（Acetylcholinesterase，AChE，EC 3.1.1.7），又称真性胆碱酯酶，能水解乙酰胆碱，发挥生理调节作用。 ② 假性胆碱酯酶（Pseudo cholinesterase，PsChE，EC 3.1.1.8），又称拟胆碱酯酶，能水解胆碱酯类，但不能水解乙酰胆碱。 AChE主要存在于神经元胞质内、神经肌肉接头（运动终板）处；PsChE主要存在于血浆、胰腺、唾液腺中，其生理功能尚不明确。测定胆碱酯酶活性可用于判断有机磷中毒程度、鉴定遗传性胆碱酯酶变异以及评估肝实质性损害程度。 本试剂盒的检测原理为：待测样品中的ChE催化乙酰胆碱水解生成胆碱和乙酸；剩余的乙酰胆碱与羟胺反应生成乙酰羟胺，后者再与三价铁离子结合生成棕色复合物。通过酶标仪检测540 nm处吸光度，根据标准曲线即可计算ChE活力。本试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
Cholinesterase (ChE) is a specific esterase that can be divided into two major categories: ① Acetylcholinesterase (AChE, EC 3.1.1.7), also known as true cholinesterase, which hydrolyzes acetylcholine and performs physiological regulatory functions. ② Pseudo cholinesterase (PsChE, EC 3.1.1.8), also known as butyrylcholinesterase, which hydrolyzes choline esters but does not hydrolyze acetylcholine. AChE is mainly present in the neuronal cytoplasm and at neuromuscular junctions (motor endplates); PsChE is mainly present in plasma, pancreas, and salivary glands, and its physiological function remains unclear. Measurement of cholinesterase activity can be used to determine the degree of organophosphate poisoning, identify hereditary cholinesterase variants, and assess the extent of hepatic parenchymal damage. The principle of this kit is as follows: ChE in the sample catalyzes the hydrolysis of acetylcholine to choline and acetic acid. The remaining acetylcholine reacts with hydroxylamine to form acetylhydroxylamine, which subsequently combines with ferric ions to form a brown complex. The absorbance is measured at 540 nm using a spectrophotometer, and the ChE activity is calculated based on a standard curve. This kit is intended for research use only and is not suitable for clinical diagnosis or other applications.
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