BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
二氧化碳（CO₂）是生物体能量代谢的重要产物之一，其含量测定在呼吸代谢研究及临床生化检测中具有广泛意义。本试剂盒采用PEPC-MDH偶联酶法测定样品中二氧化碳含量：在磷酸烯醇式丙酮酸羧化酶（PEPC）和Mg²⁺存在下，磷酸烯醇式丙酮酸（PEP）与HCO₃⁻反应生成草酰乙酸和磷酸；草酰乙酸在苹果酸脱氢酶（MDH）催化下被NADH还原为苹果酸，同时将NADH氧化为NAD⁺。在此偶联反应体系中，NADH消耗速率与样品中CO₂含量成正比，通过监测340 nm处NADH特征吸收峰的下降速率，结合标准曲线即可精准计算样品中二氧化碳的含量。
Carbon dioxide (CO₂) is one of the important products of energy metabolism in living organisms. Its quantification is of broad significance in respiratory metabolism research and clinical biochemical testing. This kit employs a PEPC-MDH coupled enzymatic method for the determination of CO₂ content in samples. In the presence of phosphoenolpyruvate carboxylase (PEPC) and Mg²⁺, phosphoenolpyruvate (PEP) reacts with HCO₃⁻ to generate oxaloacetate and phosphate. The oxaloacetate is then reduced to malate by NADH under the catalysis of malate dehydrogenase (MDH), with concomitant oxidation of NADH to NAD⁺. In this coupled reaction system, the rate of NADH consumption is directly proportional to the CO₂ content in the sample. By monitoring the rate of decrease in the characteristic absorbance peak of NADH at 340 nm and referencing a standard curve, the CO₂ content in the sample can be precisely calculated.
危险品化学品经营许可证（带存储）