计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
过氧化氢酶(Catalase, CAT, EC 1.11.1.6)是一种常见的抗氧化酶,可催化过氧化氢(H₂O₂)分解为水和氧气,普遍存在于含有细胞色素系统的好氧细胞中。过氧化氢对细胞具有高度毒性,其积累会导致DNA、蛋白质和脂质等细胞靶标发生氧化损伤,从而诱发突变和细胞死亡。过氧化氢酶通过清除细胞内的H₂O₂,防止氧化损伤的发生。目前,过氧化氢酶在氧化应激相关疾病中的作用已得到广泛研究。 本试剂盒利用过氧化氢酶的过氧化物酶功能测定其活性。在适宜浓度的H₂O₂存在下,过氧化氢酶催化甲醇反应生成甲醛;甲醛与显色剂反应生成有色产物,该产物在540 nm处具有特征吸收峰。样本中的过氧化氢酶活性与吸光度值(OD₅₄₀)成正比。
Catalase (CAT, EC 1.11.1.6) is a common antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H₂O₂) to water and oxygen. It is ubiquitously present in aerobic cells containing cytochrome systems. Hydrogen peroxide is highly toxic to cells; its accumulation can cause oxidative damage to cellular targets such as DNA, proteins, and lipids, thereby inducing mutations and cell death. Catalase prevents oxidative damage by scavenging intracellular H₂O₂. Currently, the role of catalase in oxidative stress-related diseases has been extensively studied. This kit utilizes the peroxidase activity of catalase to determine its activity. In the presence of an appropriate concentration of H₂O₂, catalase catalyzes the reaction of methanol to generate formaldehyde. The formaldehyde then reacts with a chromogenic reagent to form a colored product, which exhibits a characteristic absorbance peak at 540 nm. The catalase activity in the sample is directly proportional to the absorbance value (OD₅₄₀).