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过氧化氢酶（Catalase, CAT, EC 1.11.1.6）是生物体内关键的抗氧化酶，能高效催化过氧化氢（H₂O₂）分解为无害的水和氧气，从而保护细胞免受H₂O₂引起的氧化损伤。CAT活性是衡量机体抗氧化能力的重要指标，与细胞健康、衰老及多种病理过程密切相关。 本试剂盒的检测原理为：CAT催化H₂O₂分解为水和氧气；反应体系中未被分解的H₂O₂在特定酶和荧光探针的作用下生成荧光产物。该产物在激发波长535 nm、发射波长587 nm处的荧光强度与残留H₂O₂浓度成正比，通过检测该荧光信号即可计算CAT活性。
Catalase (CAT, EC 1.11.1.6) is a key antioxidant enzyme in living organisms that efficiently catalyzes the decomposition of hydrogen peroxide (H₂O₂) into harmless water and oxygen, thereby protecting cells from oxidative damage caused by H₂O₂. CAT activity is an important indicator of the antioxidant capacity of an organism and is closely associated with cellular health, aging, and various pathological processes. The assay principle of this kit is as follows: CAT catalyzes the decomposition of H₂O₂ into water and oxygen. The residual H₂O₂ remaining in the reaction system reacts with a specific enzyme and a fluorescent probe to generate a fluorescent product. The fluorescence intensity of this product at an excitation wavelength of 535 nm and an emission wavelength of 587 nm is directly proportional to the concentration of residual H₂O₂. By detecting this fluorescence signal, CAT activity can be calculated.
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