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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
肌酸激酶（CK，EC 2.7.3.2）主要分布于心脏、肌肉和脑等组织中，可逆催化肌酸与ATP之间的转磷酰基反应，在能量转运、肌肉收缩及ATP再生中发挥重要作用。该酶催化ATP和肌酸生成磷酸肌酸与ADP，磷酸肌酸迅速完全水解为磷酸，而ATP和ADP保持稳定。通过定磷试剂测定磷酸肌酸水解释放的磷酸量，即可计算CK的活性。
Creatine kinase (CK, EC 2.7.3.2) is predominantly distributed in tissues such as the heart, skeletal muscle, and brain. It reversibly catalyzes the phosphoryl transfer reaction between creatine and ATP, playing a crucial role in energy transport, muscle contraction, and ATP regeneration. The enzyme catalyzes the conversion of ATP and creatine to phosphocreatine and ADP. Phosphocreatine is rapidly and completely hydrolyzed to phosphate, whereas ATP and ADP remain stable. The CK activity can be calculated by determining the amount of phosphate released from the hydrolysis of phosphocreatine using a phosphate determination reagent.
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