计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
本试剂盒采用酶偶联法测定柠檬酸含量。柠檬酸裂解酶将其分解为草酰乙酸,后者在苹果酸脱氢酶催化下与NADH反应生成苹果酸和NAD⁺。于340 nm处监测NADH吸光度的减少量,该减少量与样品中柠檬酸含量成正比,据此实现定量检测。
This kit employs an enzyme‑coupled method for the determination of citric acid content. Citrate lyase cleaves citric acid to oxaloacetate, which is then reduced by NADH to malate in a reaction catalyzed by malate dehydrogenase, with concomitant oxidation of NADH to NAD⁺. The decrease in NADH absorbance is monitored at 340 nm, and this decrease is directly proportional to the citric acid content in the sample, thereby enabling quantitative detection.