BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
柠檬酸合酶（CS，EC 2.3.3.1）广泛存在于动物、植物、微生物及培养细胞的线粒体基质中，是三羧酸循环（TCA）的第一个限速酶，也是该循环的主要调控位点之一。本试剂盒的测定原理为：CS催化乙酰CoA和草酰乙酸缩合生成柠檬酰辅酶A，后者进一步水解产生柠檬酸；同时，该反应促使无色的DTNB转变成黄色的TNB，TNB在412 nm处具有特征吸收峰。通过测定412 nm处吸光度的增加速率，即可计算CS的酶活性。
Citrate synthase (CS, EC 2.3.3.1) is widely distributed in the mitochondrial matrix of animals, plants, microorganisms, and cultured cells. It is the first rate-limiting enzyme of the tricarboxylic acid (TCA) cycle and one of the major regulatory sites of this cycle. The assay principle of this kit is as follows: CS catalyzes the condensation of acetyl-CoA and oxaloacetate to form citryl-CoA, which is subsequently hydrolyzed to produce citrate. Concurrently, this reaction drives the conversion of colorless DTNB to yellow TNB, which exhibits a characteristic absorption peak at 412 nm. By measuring the rate of increase in absorbance at 412 nm, the enzyme activity of CS can be calculated.
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