计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
细胞质异柠檬酸脱氢酶(NADP-IDH,EC 1.1.1.42)是细胞质中NADPH的重要来源之一,在逆境中活性变化显著。本试剂盒采用NADPH偶联显色法快速测定NADP-IDH活力:NADP-IDH催化NADP⁺还原为NADPH,NADPH与显色剂反应生成450 nm处有最大吸收峰的有色产物,通过检测其生成速率即可计算酶活性。
Cytoplasmic isocitrate dehydrogenase (NADP‑IDH, EC 1.1.1.42) is one of the important sources of NADPH in the cytoplasm, and its activity changes significantly under stress conditions. This kit employs a NADPH‑coupled colorimetric method for rapid determination of NADP‑IDH activity. The principle is as follows: NADP‑IDH catalyzes the reduction of NADP⁺ to NADPH, which then reacts with a chromogenic reagent to produce a colored product with a maximum absorption peak at 450 nm. The enzyme activity can be calculated by measuring the rate of formation of this product.