计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
纤维素酶(CL,EC 3.2.1.4)广泛存在于细菌、真菌和动物体内,能够催化纤维素降解,是一类可广泛应用于医药、食品、纺织、环保及可再生资源利用等领域的酶制剂。本试剂盒采用蒽酮比色法测定CL的酶活性:CL催化羧甲基纤维素钠(CMC-Na)水解生成还原糖;还原糖在浓硫酸作用下脱水生成糠醛类化合物,该化合物与蒽酮试剂反应生成蓝绿色产物,该产物在620 nm处具有特征吸收峰。通过测定620 nm处吸光度的增加速率,即可定量计算CL的酶活性。
Cellulase (CL, EC 3.2.1.4) is widely distributed in bacteria, fungi and animals. It can catalyze the degradation of cellulose and represents a class of enzyme preparations with extensive applications in pharmaceuticals, food, textile, environmental protection, renewable resource utilization and other fields. This kit uses the anthrone colorimetric method to determine CL activity: CL catalyzes the hydrolysis of sodium carboxymethyl cellulose (CMC-Na) to produce reducing sugars. Under the action of concentrated sulfuric acid, reducing sugars are dehydrated to form furan compounds, which react with anthrone reagent to yield a blue-green product with a characteristic absorption peak at 620 nm. The enzymatic activity of CL can be quantitatively calculated by measuring the increasing rate of absorbance at 620 nm.