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植物GAPDH分为细胞质型与叶绿体型两类。其中，叶绿体NADPH依赖性GAPDH（EC 1.2.1.13）在卡尔文循环中催化1,3-二磷酸甘油酸生成3-磷酸甘油醛，并伴随NADPH向NADP⁺的转化。本试剂盒采用逆向催化动力学法测定该酶活性：以1,3-二磷酸甘油酸为底物，在过量NADPH存在下启动反应，利用分光光度计持续记录340 nm处吸光值的动态下降曲线。由于NADPH在340 nm具有特征摩尔消光系数（ε=6.22×10³ L·mol⁻¹·cm⁻¹），通过计算初始线性阶段的吸光值下降速率（ΔOD₃₄₀/min），即可换算得出样品中叶绿体GAPDH的酶活力单位（U）。
Plant GAPDH exists in two isoforms: cytosolic and chloroplastic. Among them, chloroplastic NADPH‑dependent GAPDH (EC 1.2.1.13) catalyzes the conversion of 1,3‑bisphosphoglycerate to glyceraldehyde‑3‑phosphate in the Calvin cycle, accompanied by the conversion of NADPH to NADP⁺. This kit employs a reverse catalytic kinetic method for the determination of this enzyme activity: using 1,3‑bisphosphoglycerate as the substrate, the reaction is initiated in the presence of excess NADPH. The dynamic decrease in absorbance at 340 nm is continuously recorded using a spectrophotometer. Since NADPH has a characteristic molar extinction coefficient at 340 nm (ε = 6.22 × 10³ L·mol⁻¹·cm⁻¹), the enzyme activity unit (U) of chloroplastic GAPDH in the sample can be calculated from the initial linear rate of decrease in absorbance (ΔOD₃₄₀/min).
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