BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
叶绿体是光合作用的细胞器，在光合作用研究中，常需要用提取的叶绿体展开下游研究工作；叶绿体分离为从植物叶片中分离完整的叶绿体提供了有效的方法，该方法包括机械式细胞壁和细胞膜破坏、过滤法细胞碎片收集和叶片组织、离心法叶绿体分离、采用分层液或梯度法法从破碎的叶绿体之中分离完整的叶绿体。
叶绿体提取试剂盒，采用易于执行的简单、成熟实验方案，节省时间， 分离出来的叶绿体可适于研究碳同化、电子流和磷酸化、代谢转运或蛋白质靶向等过程，其提取原理是研磨叶片得到的匀浆液，经过滤、离心可获得叶绿体，置于等渗缓冲液中检测其完整性，适用于从植物叶片(菠菜、豌豆、生菜、卷心菜、莙达菜和烟草等)中分离叶绿体以便进行结构和功能研究。
该试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
组分表
| 组分货号 | 组分名称 | 物理外观 | 50T | 储存 |
| C1527488A | 叶绿体裂解缓冲液 | 液体 | 250ml | 2-8℃ |
| C1527488B | 叶绿体悬浮液 | 液体 | 20ml | 2-8℃，避光 |
自备材料
1、 蒸馏水
2、 电子天平、离心管或试管、研钵或匀浆器、纱布或滤纸、低温离心机、显微镜
操作步骤(仅供参考)
1、选用生长健壮最好是连续几个晴天生长的菠菜等植物的叶片，洗涤后去除中脉，置于 0～
4℃冰箱预冷。
2、取 5ml叶绿体裂解缓冲液置于研钵或匀浆器中，置于冰箱中预冷至接近于 0℃。
3、取 2g 预冷的样品，剪碎后放入研钵中，手工快速研磨 0.5～1min(注意：不要用力过猛，无需研磨过细，以叶片磨成小块即可)，用 2～3 层纱布或滤纸过滤。
4、将滤液移入预冷的离心管中，4℃ 300g 离心 2min，弃沉淀。
5、取上清，移入预冷的离心管中，4℃ 1000g 离心 2min。
6、弃上清，沉淀即为叶绿体，加入 200～400μl 预冷的叶绿体悬浮液，悬浮后 4℃保存待用，400-600 倍显微镜下观察叶绿体形态。
注意事项
1、 选用生长健壮最好是连续几个晴天生长的植物叶片作为待分离的样本。
2、 操作全过程尽量控制在 4℃条件下。
3、 试剂开封后请尽快使用，以防影响后续实验效果。
4、 为了您的安全和健康，请穿实验服并戴一次性手套操作。
有效期
12 个月有效。低温运输，4℃保存。
Chloroplasts are photosynthetic organelles. In photosynthesis research, extracted chloroplasts are often required for downstream studies. Chloroplast isolation provides an effective approach to obtain intact chloroplasts from plant leaves. The method includes mechanical disruption of cell walls and cell membranes, filtration for removal of leaf tissue and collection of cell debris, isolation of chloroplasts by centrifugation, and separation of intact chloroplasts from broken chloroplasts using layering solution or density‑gradient methods.
The Chloroplast Extraction Kit adopts a simple, well‑established and easy‑to‑perform experimental protocol to save experimental time. The isolated chloroplasts are suitable for studies on carbon assimilation, electron flow and phosphorylation, metabolic transport, protein targeting and other processes. Its extraction principle is as follows: leaf homogenate obtained by grinding leaves is subjected to filtration and centrifugation to harvest chloroplasts, whose integrity is then assayed in isotonic buffer. This kit is applicable for isolating chloroplasts from plant leaves (such as spinach, pea, lettuce, cabbage, chard and tobacco) for structural and functional research.
This kit is for research use only. It is not intended for clinical diagnosis or any other application
| Component No. | Component Name | Appearance | 50T | Storage |
| C1527488A | Chloroplast Lysis Buffer | Liquid | 250ml | 2-8℃ |
| C1527488B | Chloroplast suspension | Liquid | 20ml | 2-8℃. Store in the dark. |
Materials to be Prepared by User
1. Distilled water
2. Electronic balance, centrifuge tubes or test tubes, mortar or homogenizer, gauze or filter paper, refrigerated centrifuge, microscope
Operating Procedures (For Reference Only)
1. Select healthy plant leaves such as spinach preferably grown under several consecutive sunny days. Wash the leaves and remove the midribs, then pre‑cool them in a refrigerator at 0‑4 ℃.
2. Transfer 5 mL of Chloroplast Lysis Buffer into a mortar or homogenizer, and pre‑cool in refrigerator to near 0 ℃.
3. Weigh 2 g pre‑cooled sample, cut into pieces and place into the mortar. Grind rapidly by hand for 0.5‑1 min. (Note: Do not grind too hard or excessively fine; it is sufficient to crush leaves into small pieces.) Filter through 2‑3 layers of gauze or filter paper.
4. Transfer the filtrate into a pre‑cooled centrifuge tube, centrifuge at 300 g for 2 min at 4 ℃, discard the pellet.
5. Transfer the supernatant to another pre‑cooled centrifuge tube, centrifuge at 1000 g for 2 min at 4 ℃.
6. Discard the supernatant; the pellet is chloroplasts. Add 200‑400 μL pre‑cooled Chloroplast Suspension Solution to resuspend the pellet. Store at 4 ℃ for subsequent use.Observe chloroplast morphology under a microscope at 400‑600‑fold magnification.
Notes
1. Use healthy plant leaves preferably grown under several consecutive sunny days as samples for isolation.
2. Perform all operations at approximately 4 ℃ as much as possible.
3. Use the reagents promptly after opening to avoid compromised experimental performance.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
Shelf‑life
Valid for 12 months. Ship under cold conditions; store at 4 ℃.
| 组分货号 | 组分名称 | 物理外观 | 50T | 储存 |
| C1527488A | 叶绿体裂解缓冲液 | 液体 | 250ml | 4℃ |
| C1527488B | 叶绿体悬浮液 | 液体 | 20ml | 4℃; 避光 |
危险品化学品经营许可证（带存储）