微生物生化反应是指用化学反应来测定微生物的代谢产物，生化反应常用来鉴别一些在形态和其它方面不易区别的微生物。因此微生物生化反应是微生物分类鉴定中的重要依据之一。常见的生化反应试验主要包括碳水化合物的生化试验、氨基酸和蛋白质的代谢试验、碳源和氮源的利用试验、酶类的代谢试验等。有些细菌具有接触酶 (或过氧化氢酶)，能催化过氧化氢产生水和初生态的氧，继而形成氧分子出现气泡。过氧化氢酶试验试剂由氧化剂、去离子水等配制而成，有一定的腐蚀性，本产品可用于细菌的过氧化氢酶试验或触酶试验。
试验方法 (仅供参考):
1、方法一：取干净的载玻片，在上面滴 1-2 滴过氧化氢酶试验试剂，挑取一环斜面培养的试验菌，与本产品混匀，若有气泡出现则为过氧化氢酶试验阳性，无气泡产生则为阴性。
2、方法二：取 2ml 过氧化氢酶试验试剂加入干净的小试管中，用细玻棒蘸取试验菌，插入试剂液面下，若有气泡产生则为阳性。
3、方法三：将 1ml 过氧化氢酶试验试剂滴加在培养物上 (菌落或菌苔上)，若有气泡产生则为阳性。
注意事项
1、本产品仅用于科研领域，不适用于临床诊断或其他用途。
2、实验菌不应培养在含有血液的培养基上，以免造成假阳性反应。
3、不能用铂环代替玻棒，铂环接触试剂可能产生气泡。
4、每次反应均应设立对照，阳性对照菌为金黄色葡萄球菌，阴性对照菌为链球菌。
5、试剂注意密闭保存，一旦开封，保质期就会大大缩短。
6、为了您的安全和健康，请穿实验服并戴一次性手套操作。
7、试剂开封后请尽快使用，以防影响后续实验效果。
Microbial biochemical reactions refer to chemical-based assays for detecting microbial metabolites. Biochemical tests are commonly used to identify microorganisms that are difficult to distinguish by morphology and other characteristics. Accordingly, microbial biochemical reactions serve as one of the critical foundations for the taxonomic identification of microorganisms. Common biochemical tests include assays for carbohydrate metabolism, amino-acid and protein metabolism, carbon-source and nitrogen-source utilization, enzyme-related metabolic tests, and so on.Certain bacteria produce catalase, which catalyzes the decomposition of hydrogen peroxide into water and nascent oxygen, subsequently forming oxygen gas and producing bubbles. The catalase test reagent is formulated with oxidants, deionized water and other ingredients and is mildly corrosive. This product is intended for the catalase test of bacteria. For research-only use. Not suitable for clinical diagnosis or other applications.
Test Procedures (For Reference Only)
1. Method 1: Place 1-2 drops of catalase test reagent onto a clean glass slide. Pick one loop of test bacteria cultured on an agar slant and mix thoroughly with the reagent. Bubble formation indicates a positive catalase-test result; the absence of bubbles indicates a negative result.
2. Method 2: Pipette 2 mL of catalase test reagent into a clean small test-tube. Use a thin glass rod to collect the test strain and submerge it beneath the liquid surface of the reagent. A bubble-generating reaction is regarded as positive.
3. Method 3: Add 1 mL of catalase test reagent onto the culture (colony or bacterial lawn). Bubble formation indicates a positive reaction.
Precautions
1. Do not culture test organisms on blood-containing media, as this may cause false-positive results.
2. Platinum loops cannot replace glass rods. Contact between platinum loops and the reagent may produce false bubbles.
3. Set up control groups for each reaction. Staphylococcus aureus is used as the positive-control strain, and Streptococcus spp. as the negative-control strain.
4. Store the reagent in a tightly-sealed container. The shelf-life will be greatly shortened once opened.
5. For your personal safety, wear a lab coat and disposable gloves during operation.
6. Use the reagent promptly after opening to avoid compromised experimental performance.
危险品化学品经营许可证（带存储）