重组羧肽酶B是一种蛋白水解酶，可特异性切割蛋白质C-末端的碱性氨基酸（赖氨酸<Lysine，K>以及精氨酸<Arginine，R>的氨基端，分子量34.7 kDa，最适pH为7.5-9.0，其活性受精氨酸和赖氨酸的竞争性抑制、金属离子螯合剂（如EDTA等）的抑制。
本品氨基酸序列与猪胰腺来源的羧肽酶B完全一致，具有与动物源性羧肽酶B相同的酶学性质。
采用低内毒素大肠杆菌CleanTAKTM菌株表达的重组羧肽酶B，内毒素含量极低且不含任何动物来源的病原体，适用范围广,特别适用于科研、胰岛素的生产、医学诊断等领域。
使用说明
溶液配制：用无菌水或者25mM Tris-HCl（pH7.6）溶解重组羧肽酶B，使酶液浓度为1-10mg/ml；
酶量：目的蛋白（质量比）=1:50-1:1000，最适pH为7.5-9.0。
溶液保存：配制的溶液可当天使用或低温2-8 ℃保存；长期保存：重组羧肽酶B酶液若需长期保存，则将重组羧肽酶B储存液分装后于-20 ℃或更低温度保存，避免冻融。
注：此产品为冻干粉，使用前建议将冻存管离心后取出蛋白使用。
产品用途
具有与动物源性羧肽酶B相同的酶学性质，可应用于各种生物技术过程：
重组胰岛素及其类似物的生产；
蛋白质C-末端氨基酸的测定；
其它重组多肽类物质的生产；
某些特殊化合物的酶促合成。
产品优势
内毒素含量极低：低内毒素大肠杆菌CleanTAKTM 菌株表达的重组羧肽酶B产品内毒素含量极低；
无动物源性：重组酶，不含任何动物来源原料；
质量稳定：批量生产，产品批次间无差异；
纯度高：微生物、宿主蛋白、外源性DNA、金属离子等的残留低于生物制品限度要求。
注意事项
酶活性受精氨酸和赖氨酸的竞争性抑制、金属离子螯合剂（如EDTA等）的抑制；
为了您的安全，请穿实验服并佩戴手套操作；
仅供科研生产使用，不能用于人体或其他用途。
Recombinant Carboxypeptidase B is a proteolytic enzyme that specifically cleaves the N‑terminus of basic amino acids (Lysine, K and Arginine, R) at the C‑terminus of proteins. It has a molecular weight of 34.7 kDa and an optimum pH of 7.5‑9.0. Its activity is competitively inhibited by arginine and lysine, and is also inhibited by metal‑ion chelators such as EDTA.
The amino‑acid sequence of this product is identical to that of porcine pancreatic‑derived carboxypeptidase B, and it exhibits identical enzymatic properties to animal‑derived carboxypeptidase B.
Expressed in the low‑endotoxin *E. coli* CleanTAK™ strain, recombinant Carboxypeptidase B features extremely low endotoxin levels and is free of any animal‑derived pathogens. It has a broad scope of applications, especially for research, insulin manufacturing, medical diagnostics and other fields.
Instructions for Use
Solution Preparation: Reconstitute recombinant Carboxypeptidase B in sterile water or 25 mM Tris‑HCl (pH 7.6) to a concentration of 1‑10 mg/mL.
Enzyme Dosage: Enzyme : target protein (mass ratio) = 1:50‑1:1000; optimum pH: 7.5‑9.0.
Solution Storage: The prepared solution may be used on the day of preparation or stored at 2‑8 °C. For long‑term storage: aliquot the reconstituted recombinant Carboxypeptidase B stock solution and store at −20 °C or lower. Avoid repeated freeze‑thaw cycles.
Note: This product is supplied as lyophilized powder. Centrifuge the vial prior to opening to collect all powder at the bottom.
Product Applications
Possessing enzymatic properties identical to animal‑derived carboxypeptidase B, it can be applied in various biotechnological processes:
‑ Production of recombinant insulin and its analogues;
‑ Determination of C‑terminal amino acids of proteins;
‑ Manufacturing of other recombinant polypeptides;
‑ Enzymatic synthesis of certain special compounds.
Product Advantages
‑ Extremely low endotoxin: Recombinant Carboxypeptidase B expressed by low‑endotoxin *E. coli* CleanTAK™ strain has extremely low endotoxin content.
‑ Animal‑free: Recombinant enzyme without any animal‑derived raw materials.
‑ Consistent quality: Mass production with negligible batch‑to‑batch variation.
‑ High purity: Residuals of microorganisms, host‑cell protein, exogenous DNA and metal ions are below the limits for biological products.
Precautions
‑ Enzyme activity is subject to competitive inhibition by arginine and lysine, and inhibition by metal‑ion chelators (e.g. EDTA).
‑ For personal safety, wear a lab coat and gloves during handling.
‑ For research and manufacturing use only. Not for human administration or any other purposes.
危险品化学品经营许可证（带存储）