Casein is considered to be a generic substrate for a broad spectrum of proteases. As native casein this fluoresceinated casein is hydrolyzed by many proteases, and widely used for fluorimetric measurement of protease activity. In the intact substrate, casein is heavily labeled with 5-FITC, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly green fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity. We do not recommend that this conjugate be used for fluorescence polarization assay. For fluorescence polarization we can custom-make the lightly labeled fluorescein casein conjugate.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the material to approximately 5 mg/ml by addition of 1mL distilled water. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C, avoid repeated freeze-thaw cycles.
储存条件
2-8°C储存,避光
运输条件
低温运输
稳定性与储存
Product should be stored at 2-8℃ in the dark. After reconstitution, store in aliquots and freeze material at -20℃, avoid freeze/thaw cycle.