L2205164计算溶液所需的质量、体积或浓度。
10×Capping Reaction Buffer
有货
为什么选择此级别
PharmPure™,医药级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
10×Capping Reaction Buffer 是牛痘病毒加帽酶（GMP-M062）和 mRNA Cap 2’-O 甲基转移酶（GMP-M072）的配套反应 缓冲液。1×Capping Reaction Buffer 配方为：50mM Tris-HCl（pH 8.0 at 25℃），5mM KCl，1mM DTT，1mM MgCl2。
本制品采用药用规格原辅料生产，并严格控制生产过程中各类污染等，符合 GMP 规范的产品生产与质量管理规程保障生 产过程及所有原辅料可追溯。
质量要求
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遵循以下规范生产
1. ISO 9001:2015, certified facility。
2. 《GMP 附录-细胞治疗产品》国家药品监督管理局。
3. 《人用基因治疗总论-中国药典 2020》国家药典委。
4. USP Chapter <1043>, Ancillary Materials for Cell, Gene, and Tissue-Engineered Products 用于细胞治疗，基因治疗和组织工程 产品中的辅料。
5. USP Chapter <92>, Growth Factors and Cytokines Used in Cell Therapy Manufacturing 细胞治疗产品生产过程中细胞因子和 生长因子。
6. Ph. Eur. General Chapter 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products 用于生产细胞或基因治疗药物的生物来源原料。
产品特点
通用性：适用于 Cap0 和 Cap1 RNA 合成的酶促加帽反应。
高效性：本体系可用于 T7 RNA Polymerase反应产生的 RNA 的加帽反应，使加帽反应在一小时之内完成，效率接近 100%，并且保证正确的方向。
保存温度
-20±5 ℃。
注意事项
1. 使用时请上下颠倒轻轻混合，避免起泡，并经轻微离心后使用。反应液的配制、分装请使用新的（无RNA 酶污染的）枪头、Microtube等，确保所有试剂中无 RNA 酶污染。
2. 为保证反应有效进行，请在冰上操作。
3. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
ten × Capping reaction buffer is a matching reaction buffer for vaccinia virus capping enzyme (gmp-m062) and mRNA cap 2 '-o methyltransferase (gmp-m072). one × The formula of capping reaction buffer is: 50mm Tris HCl (pH 8.0 at 25 ℃), 5mm KCl, 1mm DTT, 1mm MgCl2.
This product is produced with raw and auxiliary materials of medicinal specifications, and all kinds of pollution in the production process are strictly controlled. Product production and quality management procedures in line with GMP specifications ensure that the production process and all raw and auxiliary materials can be traced.
Quality requirements
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Follow the following specifications
1. ISO 9001:2015, certified facility。
2. GMP appendix - cell therapy products State Drug Administration.
3. general introduction to human gene therapy - Chinese Pharmacopoeia 2020, National Pharmacopoeia Committee.
4. USP chapter <1043>, adjuvant materials for cell, gene, and tissue engineered products.
5. USP chapter <92>, growth factors and cytokines used in cell therapy manufacturing.
6. Ph. Eur. General chapter 5.2.12, raw materials of biological origin for the production of cell-based and gene therapy medical products.
Product features
Versatility: it is applicable to the enzymatic capping reaction of cap0 and cap1 RNA synthesis.
Efficiency: this system can be used for the capping reaction of RNA produced by T7 RNA polymerase reaction, so that the capping reaction can be completed within one hour, the efficiency is close to 100%, and the correct direction can be ensured.
Storage temperature
-20±5 ℃。
Matters needing attention
1. when using, please mix it upside down gently to avoid foaming, and use it after slight centrifugation. For the preparation and sub packaging of reaction solution, please use new (non RNase contaminated) gun heads, microtubes, etc. to ensure that all reagents are free of RNase contamination.
2. to ensure effective reaction, please operate on ice.
3. for your safety and health, please wear experimental clothes and disposable gloves.
规格
别名
10×封盖反应缓冲液
规格或纯度
医药级，10×
英文名称
10×Capping Reaction Buffer
储存条件
-20°C储存
运输条件
超低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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