计算溶液所需的质量、体积或浓度。
概述
Product description：
Number of Samples
Sufficient for 25 samples
Amount of Samples
Up to 1 nmol of glycolipid
Suitable of Samples
Isolated glycolipids containing a ceramide backbone.
Suggested usage
1. Pipette 5 μl of LZ-CER-BUFFX4 into an Eppendorf tube, followed by 5 μl of approximately 1nmol of the glycolipid sample to be tested.
2. For the positive control reaction, add 5 μl of GLIP-GM1-01 substrate to 5 μl of LZ-CER-BUFFX4 into an Eppendorf tube
3. Add 2 μl of LZ-CER-HM-10 (the enzyme) to the reaction mixture and mix gently by tapping the Eppendorf tube with your finger. Make total reaction mix up to 20 μl with water.
4. Incubate your samples in a water bath, oven or any other constant heating source at 37°C for 24 hrs.
The samples are now ready for procainamide, 2-AB or 2-AA labeling and subsequent analysis by HPLC. Samples can be stored frozen at this point.
Application
Ceramide glycanase is an enzyme used to release glycans from glycosphingolipids (GSLs) to enable their characterisation. It cleaves glycans including GM1, GM2 and GM3 by cleaving the β-glycosyl linkage. Glycans can then be labelled using LudgerTag labelling technology.
规格
产品名称
Ceramide Glycanase
别名
神经酰胺聚糖酶
分子类型
小分子
产品线
生物产品
储存与运输
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
高级数据
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
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