计算溶液所需的质量、体积或浓度。
概述
Product Description
alpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, core alpha mannosidase, exo-alpha-mannosidase
The enzyme may inhibit other mannosidases, such as α(1-2,3,6) mannosidase, if a noncleavable α(1-6) mannose is present on the substrate. It should therefore always be added subsequent to digestion by other mannosidases.
Alpha-(1-6) Core Mannosidase cleaves unbranched, terminal non-reducing mannose linked alpha-(1-6) to the mannosyl chitobiose core.
Formulation
The enzyme is provided as a sterile solution in 50 mM sodium phosphate pH 7.5, 0.1 mM ZnCl2.
Contents
Core Alpha Mannosidase in 50 mM sodium phosphate, 0.1 mM ZnCl (pH 7.5).
200 µl 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0).
Purity
Each lot of α(1-6) mannosidase is tested for contaminating substances by incubating the enzyme for 24 hours at 37°C with substrates indicated in the table below. No detectable activity is evident for any of these potential contaminants. The detection limit of this assay is 5 μU/mL (IUB).
The production host strain has been extensively tested and does not produce any detectable glycosidases.
Molecular weight
~52,000 daltons
Specific Activity Assay
One unit of Alpha-(1-6) Mannosidase is defined as the amount of enzyme required to produce 2 µmoles of mannose in 1 minute at 37˚C, pH 5.0 from Alpha-(1-6) mannobiose.
Suggested usage
1. Add up to 1 nmol of oligosaccharide.
2. Add deionized water to 15 µl.
3. Add 4 µl 5x Reaction Buffer 5.0.
4. Add 1 µL of enzyme
5. Incubate ten minutes at 37˚C.
Stability
Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.
Applications
α(1-6) Core Mannosidase is useful for:
Determining mannose linkages
Rapidly removing the α(1-6) core mannose resistant to other mannosidases
规格
产品名称
Core Alpha Mannosidase
别名
核心α-甘露糖苷酶
英文别名
α-D-Mannosidase MannohydrolaseMannosidase
规格或纯度
Specific Activity ＞1.5 U/mg;Activity 1 U/ml
生物活性
1 U/ml
酶学委员会编号
3.2.1.24
分子类型
小分子
产品线
生物产品
储存与运输
浓度
Specific Activity ＞1.5 U/mg;Activity 1 U/ml
储存条件
2-8°C储存
运输条件
低温运输
高级数据
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溶液计算器
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