Chip Substrates

货号: C491347
有货
级别和纯度: spot dark
★
规格
库存
价格
数量
1ml
C491347-1ml
期货 Stock Image
¥143.90
5ml
C491347-5ml
期货 Stock Image
¥514.90
25ml
C491347-25ml
期货 Stock Image
¥1,956.90
50ml
C491347-50ml
现货 Stock Image
¥2,986.90
100ml
C491347-100ml
现货 Stock Image
¥4,428.90
250ml
C491347-250ml
现货 Stock Image
¥8,548.90
500ml
C491347-500ml
期货 Stock Image
¥12,771.90
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为什么选择此级别

,适用于对基线干扰要求严格的色谱和分析工作流程。

🌡

储存与运输

2-8°C储存,避光。低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

一、产品属性
保质期: 36个月
标记酶:辣根过氧化物酶(HRP)
反应时间: 较短(20-25°C下,可达20分钟)
批间差: < 10%
沸点:100℃(Calculated flash point)
闪点:120 °C (Calculated flash point)
酸碱度范围: 4.5-4.9 (Experimental data)
密度(20℃):1.0064 g/cm³
溶解性:易溶于水
外观:无色液体
气味:无味
对光线&热敏感

二、保存及运输条件:
1. 保持容器密闭
2. 储存温度2 °C - 8 °C,避光
3. 只保留/储存在原始容器中
4. 切勿将溢出的液体放入原容器中重复使用
5. 应远离食品和饲料
6. 在环境温度不超过 30 °C,运输时间应少于一周。
7. 该溶液在过期后仍然有效,但是灵敏度可能会降低,并可能发生沉淀。
8. 被污染或泄漏的基板溶液从损坏的瓶子不应该使用,必须销毁

三、有效成分及生化机理
柠檬酸缓冲体系(pH值4.5 - 5.0),包含以下有效成分
过氧化氢
TMB
沉淀剂
过氧化物酶催化过氧化氢的分解,从TMB获得电子(通过氧化)。TMB的氧化形成一个自由基阳离子,通过二聚化而稳定,并显示出典型的蓝色。这种蓝色阳离子通过与沉淀试剂形成不可溶的络合物而稳定下来。所以在具有辣根过氧化物酶活性的位置会出现蓝色的沉淀。这种沉淀非常细,所以即使是最小的点也可以有规律地着色。

四、生物安全信息
根据法规(EC) No 1272/2008,本产品不属于危险类别;
根据法规(EC) No 1907/2006 (REACH),无有害成分

五、主要优点
1. 快速析出
2. 对塑性表面吸附力强
3.反应时间短
4.密集的深蓝色
5. 均匀的染色模式

六、使用说明
关于分装的建议
•在没有灰尘和黑暗的房间工作。
•注意溶液不要接触到仪器金属部件(导致催化) ,硅管的封闭系统是有利的。
•仔细清洁所有的仪器和容器。
•不要用裸露的手触摸与溶液接触的仪器部分。戴无粉手套。
•立即关闭瓶子,尽量减少光线和灰尘的影响。
•使用由高密度聚乙烯或聚丙烯制成的不透光的瓶子。

七、使用的一般说明
• 只有熟悉免疫学方法基础的合格实验室工作人员才能使用该解决方案。
• 注意:底物溶液对杂质非常敏感。这就是为什么永远不要将移液器吸头浸入储存瓶中的原因。未使用的溶液永远不会返回瓶中!不要互换杯子的瓶子!
• 基板解决方案用于在玻璃或塑料表面(聚碳酸酯或聚苯乙烯)上进行仪器测量的测试系统。
• 取决于系统,在与缀合物孵育后清洗微阵列,然后用底物溶液覆盖它们就足够了。阵列可以在摇摆运动下显影,直到出现明显的颜色(大约 5 到 10 分钟)。可以使用湿阵列或在用蒸馏水洗涤并干燥后进行测量。干式微芯片可用于记录用途。 (注意:有些胶水会破坏有色沉淀物)

1、Product attribute
Shelf life: 36 months
Marker enzyme: Horseradish peroxidase (HRP)
Reaction time:short (up to 20 minutes) at 20-25°C
Lot-to-lot variation:< 10 %
Boiling point : 100℃(Calculated flash point)
Flash point::120 °C (Calculated flash point)
pH-Value (at 20 °C): 4.5-4.9 (Experimental data)
Density (20℃) : 1.0064 g/cm³
Water solubility: easily soluble
Appearance: colourless liquid
Odour: odourless
Light sensitive
Heat sensitive

2、Requirements for storage rooms and vessels
1.Keep container tightly closed.
2.Keep cool. Protect from sunlight.
3.Keep/Store only in original container.
4.Never return spills in original containers for reuse.
5. Keep away from: Food and feedingstuffs.
6. The solution can be transported at room temperature, but temperatures exceeding 30 °C have to be avoided. Shipping should be completed within one week.
7. The solution will still work beyond the expiry date, but a lower sensitivity has to be taken into account and a precipitate may occur.
8. Contaminated or leaked out substrate solution from damaged bottles should not be used and has to be destroyed.

3、Effective Components and Principle of Function
A citrate buffer system (pH 4.5 - 5.0) contains the following effective components:
Hydrogen peroxide
3,3`,5,5`-Tetramethylbenzidine (TMB)
precipitating reagent
Peroxidase catalyses the decomposition of hydrogen peroxide, taking electrons from TMB (by
oxidation). Oxidation of TMB forms a radical cation that stabilizes by dimerisation and shows the typical blue colour. This blue cation is stabilized by forming an insoluble complex with the precipitating reagent. So at positions with horseradish peroxidase activity a blue precipitate will appear. This precipitate is very fine so that even the smallest dots can be coloured regularly.

4、Biosafety information
This mixture is not classified as hazardous in accordance with Regulation (EC) No 1272/2008;
Hazardous components:none (according to Regulation (EC) No 1907/2006 (REACH))

5、Advantage
1. Rapid precipitation
2. Strong adherence to plastic surfaces
3. Short reaction time
4. Intensive dark blue colour
5. Homogeneous staining pattern

6、Instruction for usage
For bottling consider the following instructions:
•Work in a dust free and darkened room.
• Pay attention that the solution has no contact with metal parts (leading to catalysis) of your
instruments. Closed systems of silicon tubes are favoured.
• Clean all instruments and vessels very carefully.
• Never touch parts of the instruments that are in contact with the solution with the naked hand. Wear powder free gloves.
• Close the bottles immediately to minimize the influence of light and dust.
• Use bottles that are not permeable to light, made from HDPE or PP.

7. General Instructions for the Use on Microchips
• This solution should only be used by qualified laboratory staff familiar with the basics of immunological methods.
• Note: The substrate solution is very sensitive to impurities. This is why you should never immerse pipette tips in storage bottles. Unused solution is never returned to the bottle! Don't swap the bottles for the cups!
• Substrate Solutions Test systems for instrumental measurements on glass or plastic surfaces (polycarbonate or polystyrene).
• Depending on the system, it may be sufficient to wash the microarrays after incubation with the conjugate and then cover them with the substrate solution. Arrays can be developed with a rocking motion until visible color appears (approximately 5 to 10 minutes). Measurements can be performed using wet arrays or after washing with distilled water and drying. Dry microchips can be used for recording purposes. (Note: some glues will destroy colored deposits)

规格

产品名称
Chip Substrates
别名
芯片基板
规格或纯度
spot dark
分子类型
小分子
储存与运输
浓度
spot dark
储存条件
2-8°C储存,避光
运输条件
低温运输

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!

找到4个结果

批号(Lot Number) 证书类型 货号
B2308542 分析证书 C491347
B2308562 分析证书 C491347
C2511043 分析证书 C491347
J2510033 分析证书 C491347
溶液计算器