计算溶液所需的质量、体积或浓度。
C4-Deficient Guinea Pig Serum
有货
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Purity
No C4 detectable by hemolytic assay
AssaysPrecautions
Use normal precautions for handling animal blood products
General Description
C4-deficient guinea pigs were characterized in 1971 as being completely genetically and functionally deficient in C4 (Ellman, L. et al. (1970); Ellman, L. et al. (1971); Frank, M.M., et al. (1971)). This line of guinea pigs has been maintained and used extensively for the characterization of complement. In fact, these animals were a key element in the rediscovery and detailed characterization of the alternative pathway of complement (Ellman, L. et al. (1971); Frank, M.M., et al. (1971)). The product is tested for the absence of C4 by functional assays for classical pathway activity. C4-D guinea pig serum reconstituted with human C4 is certified to possess a functional classical pathway for complement activation after reconstitution (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)). The absence of C4 would also be predicted to prevent complement activation by the lectin pathway, but the function of this pathway is not tested.
Physical Characteristics
C4-D guinea pig serum is supplied as a clear, straw-colored liquid containing all proteins of normal guinea pig serum except complement component C4.
Function
The depleted serum is tested for remaining classical pathway activity by hemolytic assays using antibody-sensitized sheep erythrocytes. The depleted serum is reconstituted with human C4 and retested to verify that a functional classical and alternative pathways are restored. The Certificate of Analysis provided with each lot gives a description of the assays and specific titers for the depleted and reconstituted sera compared to normal human serum.
The unit of classical pathway activity is the CH50. A similar unit, the C4H50, is used to quantitate the activity of C4 and C4-D guinea pig serum. A C4H50 unit is the amount of functional C4 needed to lyse 50% of 3 x107 EA cells (antibody-sensitized sheep erythrocytes) when that amount of C4 is incubated with the recommended volume of C4-D guinea pig serum in GVB++ in a total volume of 500 µL for 30 min at 37℃. This amount of C4i ndicates the sensitivity of the assay for C4 which is typically less than 2 ng C4 with 10µL C4-D guinea pig serum. See the Certificate of Analysis for lot specific values.
Applications
C4-D guinea pig serum is used to assay C4 activity in samples and to supply a serum unable to activate complement via the classical pathway. Note that C1 and C2 may still be activated in the absence of C4, but whereas there is a C2 by-pass system there does not appear to be an efficient C4 by-pass mechanism. Low level lysis of EA in C4-D guinea pig serum has been shown to require activation of the early classical and the alternative pathways (Wagner, E. et al. (1999)).
Precautions/Toxicity/Hazards
The source is human serum, therefore precautions appropriate for handling any blood-derived product must be used even though the source was shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-1 and HIV-II.
Hazard Code: B WGK Germany 3
MSDS is available upon request.
规格
产品名称
C4-Deficient Guinea Pig Serum
别名
C4 缺乏豚鼠血清
规格或纯度
>30 mg protein/mL
生物活性
>1,000,000 C4H50 units/mg C4 added to assay with C4-D GPS
分子类型
血清/血浆
产品线
生物产品
储存与运输
浓度
＞30 mg protein/mL
储存条件
-80℃储存
运输条件
特低温运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）