计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
CL097, a potent TLR7 and TLR8 agonist, induces pro-inflammatory cytokines in macrophages CL097 induces NADPH oxidase priming, resulting in an increase of the fMLF-stimulated ROS production .
In Vitro
CL097 induces activation of NF-κB at 0.1 μM in TLR7 transfected HEK293 cells and at 4 μM in TLR8-transfected HEK293 cells. CL097 induces hyperactivation of the NADPH oxidase by stimulating the phosphorylation of p47phox on selective sites in human neutrophils and suggest that p38 MAPK, ERK1/2, protein kinase C, and Pin1 control this process. CL097 induces the phosphorylation of p47phox on specific sites and enhances fMLF-induced p47phox phosphorylation. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: Neutrophils Concentration: 0, 0.5, 2.5, 5, and 10 μg/mL Incubation Time: Pretreated for 30 minutes Result: Induced phosphorylation of p47phox on specific sites in a concentration-dependent manner.
In Vivo
CL097 and CD40 agonist stimulation induces efficient diabetogenic Cytotoxic T lymphocyte (CTL) function in NOD mice. CL097 (5 mg/kg, s.c.) alone causes a modest specific lysis of the target peptide (∼25%). However, treatment with a combination of CL097 and CD40 agonist (10 mg/kg, i.p.) results in an increase of approximately twofold in the specific lysis of the IGRP-peptide-coated targets compared with CL097 treatment alone. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Female 8.3 NOD mice (5-6 weeks old)Dosage: 5 mg/kg Administration: Injected s.c. Result: Caused a modest specific lysis of the target peptide (∼25%).
IC50& Target:TLR7 TLR8
规格
规格或纯度
10mM in DMSO
英文名称
CL097
生化机理
CL097 是一种强效的 TLR7 和 TLR8 激动剂，能诱导巨噬细胞中的促炎细胞因子。CL097 可诱导 NADPH 氧化酶，导致 fMLF 刺激的 ROS 生成增加。
储存条件
-80℃储存
运输条件
特低温运输
名称和识别符
分子类型
小分子
分子量
242.28
技术文档
高级数据
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
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