计算溶液所需的质量、体积或浓度。
Collagenase - Elastase blend
有货
为什么选择此级别
用于细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
The Collagenase-Elastase blend is a tissue dissociation enzyme cocktail for cell isolation. The tissue dissociation process consists in the release of functional and viable cells from animal tissue, with minimal impact on the cell’s viability. The main reagent used for the tissue dissociation process is Collagenase. Collagenases are enzymes that break down the native collagen that holds animal tissues together. The second enzyme found in our blend is Elastase: a serine protease that also hydrolyzes amides and esters. It is produced in the pancreas as an inactive zymogen and activated in the duodenum by trypsin. While elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin. Elastin, a matrix component of tissues involved in stretching processes, is found in highest concentrations in the elastic fibers of connective tissues. Elastase is frequently used to dissociate tissues which contain extensive intercellular fiber networks. For this purpose, it is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin. Elastase is the enzyme of choice for the isolation of Type II cells from the lung.
The Collagenase-Elastase blend is a tissue dissociation enzyme cocktail for cell isolation. The tissue dissociation process consists in the release of functional and viable cells from animal tissue, with minimal impact on the cell’s viability. The main reagent used for the tissue dissociation process is Collagenase. Collagenases are enzymes that break down the native collagen that holds animal tissues together. The second enzyme found in our blend is Elastase: a serine protease that also hydrolyzes amides and esters. It is produced in the pancreas as an inactive zymogen and activated in the duodenum by trypsin. While elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin. Elastin, a matrix component of tissues involved in stretching processes, is found in highest concentrations in the elastic fibers of connective tissues. Elastase is frequently used to dissociate tissues which contain extensive intercellular fiber networks. For this purpose, it is usually used with other enzymes such as collagenase, trypsin, and chymotrypsin. Elastase is the enzyme of choice for the isolation of Type II cells from the lung.规格
规格或纯度
用于细胞培养, Tissue dissociation blend, lyophilized powder
英文名称
Collagenase - Elastase blend
生化机理
胶原酶从组织中有效释放细胞需要胶原酶的作用。胶原蛋白酶由每摩尔酶中的四克原子钙（Ca2+）激活。最佳 pH 值为 6.3-8.8。这种酶通常用于消化组织样本中的结缔成分，以释放单个细胞。胶原酶处理会导致一些细胞死亡。消化时通常使用 0.1 至 5 毫克/毫升不等的浓度。反应时间从 15 分钟到数小时不等，可获得满意的细胞解离效果，但不会造成过多细胞死亡。弹性蛋白酶：水解弹性纤维的特殊蛋白质弹性蛋白，并消化血红蛋白、酪蛋白和纤维蛋白。 它能在疏水性小氨基酸（如 Ile、Gly、Ala、Ser、Val 和 Leu）的羧基侧裂解蛋白质。该酶还能水解酰胺和酯类，如 N-苯甲酰基-L-丙氨酸甲酯。最佳 pH 值为 8.0-8.5。它不需要任何激活剂，但会受到氟磷酸二异丙酯、苯甲基磺酰氟、α2-巨球蛋白、α1-抗胰蛋白酶、磺酰氟、对二硝基苯二乙基磷酸酯和高浓度盐的抑制。 它能在疏水性小氨基酸（如 Ile、Gly、Ala、Ser、Val 和 Leu）的羧基侧裂解蛋白质。该酶还能水解酰胺和酯类，如 N-苯甲酰基-L-丙氨酸甲酯。
储存条件
-20°C储存
运输条件
超低温运输
名称和识别符
分子类型
酶
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
常见问题
什么是「用于细胞培养」？
「用于细胞培养」表示该产品经过加工和检测，适用于细胞培养和组织工程。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于?20 °C条件下保存。需冷冻保存以维持规定的有效期。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）