α-Chymotrypsin agarose from bovine pancreas has been used to study the purification and characterization of glucoamylase. α-Chymotrypsin agarose from bovine pancreas has also been used in a study to investigate molecular modeling for the design of a biomimetic chimeric ligand.
α-Chymotrypsin agarose from bovine pancreas has been used to study the purification and characterization of glucoamylase. α-Chymotrypsin agarose from bovine pancreas has also been used in a study to investigate molecular modeling for the design of a biomimetic chimeric ligand.
规格
规格或纯度
lyophilized powder, 2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)
英文名称
α-Chymotrypsin−Agarose from bovine pancreas
生化机理
α-糜蛋白酶是一种丝氨酸肽酶,有 241 个氨基酸残基,包含在由二硫桥连接的三条多肽链中(A 链-13 个残基、B 链-131 个残基和 C 链-97 个残基)。这种酶的分子量为 25 kDa。pI 为 8.75。它能选择性地水解酪氨酸、苯丙氨酸、色氨酸和亮氨酸 C 端侧的肽键。Ca2+可激活并稳定该酶。氟磷酸二异丙酯(DFP)、苯甲基磺酰氟(PMSF)、N-对甲苯磺酰基-L-苯丙氨酸氯甲基酮(TPCK)、糜蛋白酶、阿普汀、α1-抗胰蛋白酶、α2-巨球蛋白、10 mMCu2+和Hg2+均可抑制该酶。丝氨酸蛋白酶,可水解肽键羧基末端带有芳香族或大疏水侧链(Tyr、Trp、Phe、Met)的肽键。