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微生物来源的硫酸软骨素（CS）/硫酸皮肤素（DS）糖链降解酶（CSases）属于多糖裂解酶，通过b-消除反应使N-乙酰半乳糖胺（GalNAc）与己糖醛酸（GlcUA/IdoUA）之间的β-1,4糖苷键断裂，同时在糖醛酸的C4和C5位碳原子之间形成不饱和双键，该双键在232 nm有特征吸收，可以方便地用于寡糖产物分析检测。商品化的CSases包括：来自Proteus vulgaris的CSase ABC，该酶可以同时降解CS和DS及HA，实际上CSase ABC是两个酶的混合物，其中CSase ABCI是一个CS/DS内切酶，而CSase ABCII则是一个CS/DS的非还原端外切酶；来自Flavobacterium heparinum的CSase ACI和B，其中CSase ACI是CS和HA专一性内切酶，而后者则是DS专一性内切酶；而来自Arthrobacter aurescens的CSase ACII是另一个CS和HA专一性降解酶，但该酶是一个外切酶，在CS寡糖还原端被荧光标记后，该酶可以有效切割还原端标记四糖的酶，因此在CS寡糖酶解测序中特别有用。CS/DS裂解酶不仅是研究CS/DS构效关系和制备CS/DS寡糖的重要工具酶，也在中枢神经系统损伤治疗中有着重要的临床应用价值。我们可以根据客户需要为客户提供不同底物选择性、不同底物降解模式和不同规格的各种CSases，满足CS/DS结构功能分析、产品质量检测、肝素/硫酸乙酰肝素生产纯化、CS和DS功能寡糖大规模酶解制备等各种需求。
Microbial derived chondroitin sulfate (CS)/dermatan sulfate (DS) sugar chain degrading enzymes (CSases) belong to polysaccharide lyases, which break the β -1,4-glycosidic bond between N-acetylglucosamine (GalNAc) and hexuronic acid (GlcUA/IdoUA) through a b-elimination reaction. At the same time, unsaturated double bonds are formed between the C4 and C5 carbon atoms of the uronic acid, which have characteristic absorption at 232 nm and can be conveniently used for oligosaccharide product analysis and detection. Commercialized CSases include CSase ABC from Proteus vulgaris, which can simultaneously degrade CS, DS, and HA. In fact, CSase ABC is a mixture of two enzymes, with CSase ABCI being a CS/DS endonuclease and CSase ABCII being a non reducing end exonuclease of CS/DS; CSase ACI and B from Flavobacterium heparinum, where CSase ACI is a CS and HA specific endonuclease, while the latter is a DS specific endonuclease; The CSase ACII from Arthrobacter auricens is another CS and HA specific degrading enzyme, but it is an exonuclease that can effectively cleave the enzyme labeled with tetrasaccharides at the reducing end of CS oligosaccharides after being fluorescently labeled. Therefore, it is particularly useful in CS oligosaccharides enzymatic sequencing. CS/DS lyase is not only an important tool enzyme for studying the structure-activity relationship of CS/DS and preparing CS/DS oligosaccharides, but also has significant clinical application value in the treatment of central nervous system injuries. We can provide customers with various CSases with different substrate selectivity, substrate degradation modes, and specifications according to their needs, meeting various needs such as CS/DS structural and functional analysis, product quality testing, heparin/heparan sulfate production and purification, and large-scale enzymatic hydrolysis preparation of CS and DS functional oligosaccharides.
H2505269
危险品化学品经营许可证（带存储）