The HeLa whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.
规格
产品名称
HeLa Whole Cell Lysate
别名
HeLa 全细胞裂解物
规格或纯度
Carrier Free, Azide Free, 200μg, Mycoplasma free
分子类型
细胞或组织提取物
产品线
biological_products
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4).
浓度
200μg, Mycoplasma free
复溶
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
储存条件
-80℃储存
运输条件
特低温运输
稳定性与储存
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.
图片
HeLa Whole Cell Lysate (cl155894) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa Observed band size: 42 kDa
HeLa Whole Cell Lysate (cl155894) - Western Blot All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa Observed band size: 29 kDa
HeLa Whole Cell Lysate (cl155894) - Western Blot All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906) Lane 2: MCF7 Whole Cell Lysate (cl155899) Lane 3: HeLa Whole Cell Lysate (cl155894) Lane 4: Hep G2 Whole Cell Lysate (cl155896) Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa Observed band size: 58 kDa
ZJ23F0500180
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