TF-1 erythroblasts are isolated from bone marrow derived from a 35-year-old Asian male with severe pancytopenia in 1987. The cells provide a good system for investigating the proliferation and differentiation of myeloid progenitor cells.TF-1 cells exhibit responsiveness to many other lymphokines and cytokines, including IL-1, IL-4, IL-6, IL-9, IL-11, IL-13, SCF, LIF, and NGF. They don't express blood group antigen A or carbonic anhydrase I. Morphological and cytochemical properties, as well as the expression of globin genes, suggest that they are of erythroid lineage. Hematin and delta-aminolevulinic acid induce hemoglobin synthesis in TF-1 cells, while TPA induces significant differentiation of TF-1 cells into macrophage-like cells. TF-1 cells are unique due to their responsiveness to multiple cytokines and providing a good system for detecting bone marrow progenitor cells.
规格
产品名称
TF-1 Whole Cell Lysate
别名
TF-1全细胞裂解物
规格或纯度
Mycoplasma free
分子类型
细胞或组织提取物
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na₃VO₄).
防腐剂
No
复溶
Recommended reconstitute at 1-2 mg/mL in ddH₂O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.