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SK-N-SH Whole Cell Lysate

Name: SK-N-SH Whole Cell Lysate
SKU: cl185705
Availability: InStock

货号: cl185705

COA

有货

级别和纯度: 无支原体 ?

规格

库存

价格

数量

200μg

cl185705-200μg

期货

¥2,199.90

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概述规格储存与运输图片技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 溶液计算器常见问题

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为什么选择此级别

无支原体级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-80℃储存。特低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述

This cell line is used in studies of apoptosis and neuroprotection as an in vitro model of human neuronal cells.

规格

产品名称

SK-N-SH Whole Cell Lysate

别名

SK-N-SH全细胞裂解物 | SK-N-SH 全细胞裂解物

规格或纯度

Mycoplasma free

分子类型

细胞或组织提取物

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

储存缓冲液

Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na₃VO₄).

防腐剂

复溶

Recommended reconstitute at 1-2 mg/mL in ddH₂O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.

储存条件

-80℃储存

运输条件

特低温运输

稳定性与储存

用干冰运输。冻干粉建议在 -80℃ 下保存。复溶后，建议等分，避免反复冻融，短期保存在 -20℃ 下，长期保存在 -80℃ 下。

图片

Western Blot: SK-N-SH Whole Cell Lysate (cl185705)

SK-N-SH Whole Cell Lysate (cl185705) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/2000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: HeLa Whole Cell Lysate (cl155894)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: SK-N-SH Whole Cell Lysate (cl185705)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 21.1 s

Western Blot: SK-N-SH Whole Cell Lysate (cl185705)

SK-N-SH Whole Cell Lysate (cl185705) - Western Blot
All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: HeLa Whole Cell Lysate (cl155894)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: SK-N-SH Whole Cell Lysate (cl185705)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 31 kDa
Exposure time: 113.4 s

Western Blot: SK-N-SH Whole Cell Lysate (cl185705)

SK-N-SH Whole Cell Lysate (cl185705) - Western Blot
All lanes: COX IV Mouse mAb (Ab097414) at 1/2000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: HeLa Whole Cell Lysate (cl155894)
Lane 3: LNCaP Whole Cell Lysate (cl155902)
Lane 4: SK-N-SH Whole Cell Lysate (cl185705)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa
Exposure time: 22.9 s