U-2 OS is a cell line with epithelial morphology that was derived in 1964 from a moderately differentiated sarcoma of the tibia of a 15-year-old, White, female osteosarcoma patient.
规格
产品名称
U-2 OS Whole Cell Lysate
别名
U-2 OS全细胞裂解物 | U-2 OS 全细胞裂解物
规格或纯度
Mycoplasma free
分子类型
细胞或组织提取物
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na₃VO₄).
防腐剂
No
复溶
Recommended reconstitute at 1-2 mg/mL in ddH₂O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
U-2 OS Whole Cell Lysate (cl185708) - Western Blot All lanes: beta Actin Mouse mAb (Ab155822) at 1/2000 dilution Lane 1: U- 2 OS Whole Cell Lysate (cl185708) Lane 2: THP-1 Whole Cell Lysate (cl185703) Lane 3: MOLT-4 Whole Cell Lysate (cl155905) Lane 4: Jurkat Whole Cell Lysate (cl155897) Lane 5: HeLa Whole Cell Lysate (cl155894) Lane 6: THP-1 Whole Cell Lysate (cl185703) Lane 7: HUVEC Whole Cell Lysate (cl185700) Lane 8: TF-1 Whole Cell Lysate (cl185701) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 41 kDa Exposure time: 53.8 s
U-2 OS Whole Cell Lysate (cl185708) - Western Blot All lanes: Recombinant PCNA Antibody (Ab120681) at 1/2000 dilution Lane 1: U- 2 OS Whole Cell Lysate (cl185708) Lane 2: THP-1 Whole Cell Lysate (cl185703) Lane 3: MOLT-4 Whole Cell Lysate (cl155905) Lane 4: Jurkat Whole Cell Lysate (cl155897) Lane 5: HeLa Whole Cell Lysate (cl155894) Lane 6: THP-1 Whole Cell Lysate (cl185703) Lane 7: HUVEC Whole Cell Lysate (cl185700) Lane 8: TF-1 Whole Cell Lysate (cl185701) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 31 kDa Exposure time: 20.0 s
U-2 OS Whole Cell Lysate (cl185708) - Western Blot All lanes: Recombinant Hsp90 Antibody (Ab108929) at 1/2000 dilution Lane 1: U- 2 OS Whole Cell Lysate (cl185708) Lane 2: THP-1 Whole Cell Lysate (cl185703) Lane 3: MOLT-4 Whole Cell Lysate (cl155905) Lane 4: Jurkat Whole Cell Lysate (cl155897) Lane 5: HeLa Whole Cell Lysate (cl155894) Lane 6: THP-1 Whole Cell Lysate (cl185703) Lane 7: HUVEC Whole Cell Lysate (cl185700) Lane 8: TF-1 Whole Cell Lysate (cl185701) Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 90 kDa Observed band size: 90 kDa Exposure time: 53.5 s
危险品化学品经营许可证（带存储）