HEL cells are a lymphoblast-like cell line derived from a 30-year-old Caucasian male with malignant erythroleukemia. These cells naturally produce and can be induced to synthesize immunoglobulins. HEL cells are negative for EB virus nuclear antigen, don't express surface immunoglobulins or cytoplasmic immunoglobulins. They express HLA antigens (HLA-A3, AW32, BW35), beta-2 microglobulin, and some cells also express La antigen. HEL cells provide a model for studying erythroid differentiation and immunoglobulin gene expression, resembling hemophilia in mice.
规格
产品名称
HEL Whole Cell Lysate
别名
HEL全细胞裂解物
规格或纯度
Mycoplasma free
分子类型
细胞或组织提取物
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na₃VO₄).
防腐剂
No
复溶
Recommended reconstitute at 1-2 mg/mL in ddH₂O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.