RAW 264.7 is an adherent cell line isolated from a mouse tumor that was induced by Abelson murine leukemia virus. This cell line, with macrophage differentiation, can be used in oxidative stress, inflammatory, and antibacterial activity studies. RAW 264.7 is tumorigenic and is widely used in cancer and drug development research. The RAW 264.7 Whole Cell Lysate in was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
规格
产品名称
RAW 264.7 Whole Cell Lysate
别名
RAW 264.7全细胞裂解物
规格或纯度
无载体, 无叠氮钠, 无支原体, 200μg
分子类型
细胞或组织提取物
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4).
防腐剂
No
浓度
200μg
复溶
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
储存条件
-80℃储存
运输条件
特低温运输
稳定性与储存
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.