ATDC5 mouse embryonic teratoma cells are a cell line isolated by Atsumi et al. in 1990 from the differentiated culture of AT805 teratocarcinoma cells. During the growth phase, they exhibit many fibroblast-like phenotypes. The addition of 10 pg/ml insulin to the culture medium can induce ATDC5 cells to differentiate into chondrocytes. After reaching 100% confluence, the cells continue to grow and form cartilage nodule-like cell aggregates.
The ATDC5 Whole Cell Lysate in was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
规格
产品名称
ATDC5 Whole Cell Lysate
别名
ATDC5全细胞裂解物
规格或纯度
无叠氮钠, 无载体, 无支原体
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4).
复溶
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
储存条件
-80℃储存
运输条件
特低温运输
稳定性与储存
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.