MDA-MB-231 is an epithelial-like cell that was isolated from the mammary gland of a 40-year-old White female with adenocarcinoma. The cell can be used in assay development. The cell line can be used in testing and calibration in ISO 17025-accredited laboratories, to challenge assay performance, validate or compare test methods, and establish sensitivity, linearity, and specificity during assay validation or implementation. The MDA-MB-231 Whole Cell Lysate in was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
规格
产品名称
MDA-MB-231 Whole Cell Lysate
别名
MDA-MB-231全细胞裂解物
规格或纯度
无叠氮钠, 无载体, 无支原体
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4).
复溶
Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
储存条件
-80℃储存
运输条件
特低温运输
稳定性与储存
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.