HL-60 is a suspension cell line established from peripheral blood via leukapheresis from a patient with acute promyelocytic leukemia. These cells are promyelocytes but depending on culture conditions, HL-60 can differentiate into different lineages of mature myeloid cells; this makes it a useful model for immune response studies. Tumorigenic and of the blood, these cells are widely used in cancer research and drug development. The HL-60 Whole Cell Lysate in was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process.
规格
产品名称
HL-60 Whole Cell Lysate
别名
HL-60全细胞裂解物
规格或纯度
无载体, 无叠氮钠, 无支原体, 200μg
种属
人(Human)
分子类型
细胞或组织提取物
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na₃VO₄).
防腐剂
No
浓度
200μg
复溶
Recommended reconstitute at 1-2 mg/mL in ddH₂O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
储存条件
-80℃储存
运输条件
特低温运输
稳定性与储存
Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage.