计算溶液所需的质量、体积或浓度。
免疫（共）沉淀实验服务
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概述
专业免疫共沉淀实验服务——解锁蛋白质相互作用的金钥匙
免疫共沉淀是一项成熟且强大的技术，用于在天然或近天然条件下研究蛋白质之间的相互作用。我们提供专业、高效、可靠的免疫共沉淀 及染色质免疫共沉淀 一站式实验服务，致力于为您在生命科学基础研究、药物靶点验证、信号通路解析等领域提供关键性的实验数据支持。
我们的核心优势在于：经验丰富的技术团队、优化的实验体系、严格的质控标准以及全方位的项目支持，确保为您交付真实、可信、可重复的高质量结果。
一、 核心服务项目
我们提供两大类主流的免疫沉淀技术服务：
1. 常规免疫共沉淀服务
主要用于研究溶液状态下蛋白质与蛋白质之间的相互作用。
· 基础Co-IP服务： 验证两个或多个已知/疑似蛋白是否存在直接或间接的相互作用。
· 内源性Co-IP服务： 使用针对内源性靶蛋白的特异性抗体，在不对细胞进行任何遗传修饰的情况下，直接捕获天然状态下的蛋白复合物，结果更接近生理真实情况。（推荐首选）
· 外源性/过表达Co-IP服务： 当抗体特异性不佳或蛋白表达量过低时，通过细胞转染带标签（如FLAG, HA, Myc等）的质粒，利用标签抗体进行沉淀，成功率高，灵活性好。
· Pull-down服务：
o GST Pull-down： 用于验证蛋白质的直接相互作用，确定相互作用结构域。
o His/其它标签 Pull-down： 用于体外验证结合。
2. 染色质免疫共沉淀服务
主要用于研究在活细胞状态下，蛋白质（特别是转录因子、组蛋白修饰等）与DNA的特定序列之间的相互作用。
· 常规ChIP服务： 检测特定蛋白在基因组某个位点上的结合情况。
· ChIP-seq服务（高级选项）： 与高通量测序技术结合，在全基因组范围内筛选与目标蛋白结合的所有DNA序列，用于发现新的调控位点。二、 为什么选择我们？
1. 专业可靠，经验丰富
· 资深团队： 由分子生物学、细胞生物学领域的博士和硕士组成的项目团队，具备多年蛋白相互作用研究经验。
· 项目优化： 我们针对不同蛋白特性（如溶解度、丰度、结合强度）提供个性化的实验方案优化，攻克难啃靶点。
· 抗体经验库： 我们积累了多种靶蛋白抗体的使用经验，能为您的项目推荐并验证最有效的抗体，节省您的时间与成本。
2. 全程质控，数据真实
· 严格的阴性/阳性对照： 每个实验均设立IgG对照、Input对照等，确保结果的特异性与可重复性。
· 多重验证： 结果通过Western Blot等多种方法进行交叉验证，提供确凿的证据。
· 原始数据提供： 我们承诺提供所有Western Blot、ChIP-qPCR等原始图片和数据，确保实验过程的透明与可追溯。
3. 高效省时，成本优化
· 周期明确： 从收到样品到交付最终报告，常规Co-IP服务周期通常为 2-4周，ChIP服务周期为 3-5周。
· 一站式服务： 您只需提供实验目的、细胞系/组织样本及靶点信息，其余工作（细胞培养、质粒构建、转染、IP、WB等）均由我们完成。
· 规模效应降低成本： 我们集中采购优质试剂，建立标准化流程，为您提供更具性价比的服务价格。
4. 全方位客户支持
· 免费咨询与方案设计： 在项目开始前，我们的科学家将与您深入沟通，共同设计最佳实验路线。
· 定期进度汇报： 实验关键节点向您汇报进展，让您随时掌握项目动态。
· 详尽的结题报告： 最终交付包含完整实验步骤、清晰的实验结果图片、专业的数据分析和明确的结论说明。
三、 标准服务流程
1. 项目咨询与方案制定： 您提交需求，我们共同确认实验方案、交付标准及时间。
2. 样本与材料寄送： 您提供细胞株、组织样本或相关质粒；或由我们代购。
3. 细胞培养与处理： 我们进行规范的细胞培养、转染（如需要）及处理。
4. 蛋白样品制备： 使用温和的裂解液制备细胞/组织总蛋白，保持蛋白相互作用的完整性。
5. 免疫沉淀反应： 将抗体与琼脂糖珠耦联，与蛋白样品孵育，捕获目标蛋白及其复合物。
6. 洗涤与洗脱： 彻底去除非特异性结合，获得纯净的蛋白复合物。
7. 结果检测与分析：
o Co-IP： 通过Western Blot检测共沉淀的蛋白。
o ChIP： 通过qPCR或测序检测富集的DNA片段。
8. 数据交付与售后： 交付完整结题报告，并提供专业的数据解读支持。
四、 客户需提供的信息与材料
· 实验目的： 明确需要验证的蛋白相互作用或DNA-蛋白结合。
· 靶点信息： 目标蛋白的基因名、UniProt ID等。
· 样本：
o 细胞系（推荐由我们代培养）或冻存的细胞。
o 组织样本（需提供保存条件）。
o 如需过表达，需提供相关质粒或基因序列。
· 抗体（可选）： 如果您有特定品牌的抗体要求，可自行提供或委托我们代购。
五、 典型应用场景
· 信号通路研究： 验证信号转导通路中上下游蛋白的相互作用。
· 蛋白复合物鉴定： 发现与目标蛋白结合的新伙伴。
· 药物作用机制研究： 探究小分子药物是否影响特定蛋白复合物的形成。
· 转录调控机制： 通过ChIP研究转录因子与启动子/增强子的结合。
· 表观遗传学： 研究组蛋白修饰在特定基因位点的分布。
· 疾病生物标志物发现： 筛选与疾病相关的异常蛋白相互作用。
我们的承诺
我们深知，一个可靠的蛋白相互作用结果是您课题推进的基石。我们承诺以科学的态度、严谨的操作和高效的服务，为您提供最真实、最可信的实验数据，成为您最值得信赖的科研合作伙伴。
立即联系我们，获取免费的项目评估与报价！
Professional Co-Immunoprecipitation (Co-IP) Service – Your Golden Key to Unlocking Protein Interactions
Co-immunoprecipitation (Co-IP) is a mature and powerful technique used to study protein-protein interactions under native or near-native conditions. We provide professional, efficient, and reliable one-stop experimental services for Co-IP and Chromatin Immunoprecipitation (ChIP), dedicated to delivering critical experimental data support for your work in basic life science research, drug target validation, signaling pathway analysis, and more.
Our core advantages lie in our experienced technical team, optimized experimental protocols, strict quality control standards, and comprehensive project support, ensuring the delivery of authentic, reliable, and reproducible high-quality results.
I. Core Services
We offer two main types of immunoprecipitation技术服务:
1. Conventional Co-Immunoprecipitation Services
Primarily used to study protein-protein interactions in solution.
· Basic Co-IP Service: Validates whether two or more known/suspected proteins interact directly or indirectly.
· Endogenous Co-IP Service: Uses specific antibodies against endogenous target proteins to directly capture protein complexes in their native state without any genetic modification of cells, providing results closer to the physiological reality. (Recommended as the first choice)
· Exogenous/Overexpression Co-IP Service: When antibody specificity is poor or protein expression is low, plasmids with tags (e.g., FLAG, HA, Myc) are transfected into cells, and tag antibodies are used for precipitation. This offers high success rates and good flexibility.
· Pull-down Services:
o GST Pull-down: Used to verify direct protein interactions and identify interaction domains.
o His/Other Tag Pull-down: Used for in vitro binding validation.
2. Chromatin Immunoprecipitation Services
Primarily used to study interactions between proteins (especially transcription factors, histone modifications, etc.) and specific DNA sequences in living cells.
· Conventional ChIP Service: Detects the binding of a specific protein to a particular genomic locus.
· ChIP-seq Service (Advanced Option): Combined with high-throughput sequencing technology, this screens the entire genome for all DNA sequences bound by the target protein, useful for discovering new regulatory sites.
II. Why Choose Us?
1. Professional & Reliable, Rich Experience
· Expert Team: Our project team consists of PhDs and Masters in molecular and cell biology with years of experience in protein interaction research.
· Project Optimization: We provide personalized experimental protocol optimization based on different protein characteristics (e.g., solubility, abundance, binding strength) to tackle challenging targets.
· Antibody Experience Database: We have accumulated experience using antibodies for various target proteins and can recommend and validate the most effective antibodies for your project, saving you time and cost.
2. Full-Process Quality Control, Authentic Data
· Strict Negative/Positive Controls: Each experiment includes IgG controls, Input controls, etc., to ensure specificity and reproducibility of results.
· Multiple Verification: Results are cross-verified by multiple methods like Western Blot to provide solid evidence.
· Raw Data Provision: We commit to providing all raw images and data (e.g., Western Blot, ChIP-qPCR), ensuring transparency and traceability of the experimental process.
3. Efficient & Time-Saving, Cost-Optimized
· Clear Timelines: From sample receipt to final report delivery, standard Co-IP service typically takes 2-4 weeks, and ChIP service takes 3-5 weeks.
· One-Stop Service: You just need to provide the experimental objective, cell line/tissue samples, and target information. We handle the rest (cell culture, plasmid construction, transfection, IP, WB, etc.).
· Economies of Scale Reduce Costs: We centrally procure high-quality reagents and use standardized protocols to offer more cost-effective service prices.
4. Comprehensive Customer Support
· Free Consultation & Protocol Design: Before the project starts, our scientists will communicate with you in-depth to co-design the optimal experimental plan.
· Regular Progress Updates: We report progress at key experimental stages, keeping you informed.
· Detailed Final Report: The final deliverable includes complete experimental procedures, clear result images, professional data analysis, and clear conclusions.
III. Standard Service Process
1. Project Consultation & Protocol Finalization: You submit requirements; we jointly confirm the experimental plan, deliverables, and timeline.
2. Sample & Material Shipping: You provide cell lines, tissue samples, or relevant plasmids; or we can procure them for you.
3. Cell Culture & Treatment: We perform standardized cell culture, transfection (if needed), and treatment.
4. Protein Sample Preparation: Cell/tissue total protein is prepared using gentle lysis buffers to preserve protein interactions.
5. Immunoprecipitation Reaction: Antibodies are coupled to agarose beads and incubated with the protein sample to capture the target protein and its complexes.
6. Washing & Elution: Non-specific binding is thoroughly removed to obtain pure protein complexes.
7. Result Detection & Analysis:
o Co-IP: Co-precipitated proteins are detected by Western Blot.
o ChIP: Enriched DNA fragments are detected by qPCR or sequencing.
8. Data Delivery & After-Sales Service: We deliver the complete final report and provide professional data interpretation support.
IV. Information & Materials Required from Clients
· Experimental Objective: Clearly state the protein interaction or DNA-protein binding to be validated.
· Target Information: Gene name, UniProt ID, etc., of the target protein(s).
· Samples:
o Cell lines (we recommend we culture them) or frozen cells.
o Tissue samples (please provide storage conditions).
o If overexpression is needed, provide relevant plasmids or gene sequences.
· Antibodies (Optional): If you have specific antibody brand requirements, you can provide them or entrust us to purchase them.
V. Typical Application Scenarios
· Signaling Pathway Research: Verifying interactions between upstream and downstream proteins in a signaling pathway.
· Protein Complex Identification: Discovering new binding partners of a target protein.
· Drug Mechanism of Action Studies: Investigating whether small molecule drugs affect the formation of specific protein complexes.
· Transcriptional Regulation Mechanisms: Studying transcription factor binding to promoters/enhancers via ChIP.
· Epigenetics: Researching the distribution of histone modifications at specific gene loci.
· Disease Biomarker Discovery: Screening for abnormal protein interactions associated with diseases.
Our Commitment
We understand that reliable protein interaction results are the cornerstone for advancing your research. We are committed to providing you with the most authentic and trustworthy experimental data through a scientific approach, rigorous procedures, and efficient service, aiming to be your most reliable research partner.
Contact us now for a free project evaluation and quote!
规格
别名
免疫沉淀，免疫共沉淀，IP，Co-IP，Immune (co) precipitation，Immuneprecipitation
英文别名
IP，Co-IP，Immune (co) precipitation，Immuneprecipitation
英文名称
Co-IP/IP services
高级数据
质检证书(CoA,COO,BSE/TSE 和分析图谱)
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溶液计算器
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