计算溶液所需的质量、体积或浓度。
,适用于对基线干扰要求严格的色谱和分析工作流程。
。 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
专业抗体与蛋白标记服务——为您的科研发现点亮明灯
服务概述
我们提供一站式的、高质量的抗体与蛋白标记技术服务。利用先进的标记技术和严格的质量控制体系,我们将各种功能性标记物(如荧光染料、酶、生物素、同位素等)精准地偶联到您的抗体或蛋白质上,从而赋予其“可见”或“可检测”的特性。我们的服务旨在帮助您在免疫学、细胞生物学、药物开发及体外诊断等领域的研究中,获得更灵敏、更准确、更可靠的数据。
我们的核心标记技术平台
我们拥有多元化的标记技术,以满足不同应用的需求:
1. 荧光标记
o 标记物: FITC, TRITC, Cy3, Cy5, Cy7, AF系列 (如488, 555, 647), APC, PE, 以及近红外染料等。
o 优势: 高荧光亮度、卓越的光稳定性,适用于多重检测。
o 技术要点: 我们优化反应条件,确保标记效率高且对蛋白活性的影响最小。
2. 酶标记
o 标记物: 辣根过氧化物酶,碱性磷酸酶,β-半乳糖苷酶等。
o 优势: 信号放大作用强,灵敏度极高,是ELISA、Western Blot和免疫组化的理想选择。
o 技术要点: 精确控制酶与抗体的比例,确保高催化活性和低背景。
3. 生物素标记
o 标记物: 生物素。
o 优势: 可通过链霉亲和素进行多级信号放大,极大提高检测灵敏度。生物素-亲和素系统是目前最强大的检测系统之一。
o 技术要点: 控制生物素化程度,避免因标记过多而导致的空间位阻。
4. 其他特殊标记
o 标记物: 小分子药物、DNA条形码、纳米颗粒、金属标签等。
o 应用: 满足ADC药物开发、多重检测、质谱流式等前沿领域的需求。
我们的服务流程
我们致力于提供简单、高效、可追溯的服务体验:
1. 项目咨询与方案设计: 您提供目标蛋白信息及实验目的,我们的专家团队为您推荐最合适的标记方案和标记物。
2. 样品提交: 您提供纯化后的抗体或蛋白质样品(并提供浓度、缓冲液成分等信息)。
3. 标记与纯化:
o 活化与偶联: 使用优化的化学方法进行特异性偶联。
o 纯化: 采用凝胶过滤层析或透析等方法,彻底去除未反应的游离标记物。
4. 质量控制与分析:
o 标记效率评估: 使用紫外-可见分光光度法计算标记物与蛋白的偶联比率。
o 纯度分析: 通过SDS-PAGE或HPLC验证产物纯度。
o 活性验证: 通过ELISA、Flow Cytometry等功能实验验证标记后蛋白的生物学活性(可选)。
5. 产品交付: 最终为您提供标记好的成品、完整的质检报告以及产品使用说明。
为什么选择我们的服务?
· 卓越的专业经验: 拥有多年经验的团队,成功交付数千个标记项目,涵盖各种稀有和小量蛋白。
· 灵活与定制化: 我们不仅提供标准标记服务,更擅长根据您的特殊需求,提供完全定制化的标记解决方案。
· 严格的质量控制: 每一批产品都经过严格的质量检测,并提供详尽的质检报告,确保结果的可靠性与可重复性。
· 高活性保持: 我们优化的标记工艺能最大限度地保持抗体/蛋白的原始亲和力与生物活性。
· 快速交付周期: 标准服务通常在 1-2周 内完成,助您加速科研进程。
· 保密与安全: 我们对客户提供的样品及项目信息严格保密。
产品应用领域
我们的标记产品广泛应用于:
· 流式细胞术: 细胞表面及胞内抗原的多色分析。
· 免疫荧光与免疫组化: 组织与细胞中靶标蛋白的定位与定量。
· ELISA / 化学发光: 高灵敏度的蛋白定量检测。
· Western Blot: 蛋白表达的定性与半定量分析。
· 蛋白质芯片与生物传感: 高通量蛋白相互作用研究。
· 药物靶点发现与ADC开发: 抗体药物偶联物的前期研究。
客户须知
· 样品要求: 请提供高纯度(>90%)、高浓度、缓冲液清洁(避免含氨基类物质如Tris、甘氨酸等)的抗体/蛋白样品。
· 信息提供: 请尽可能告知我们您的实验应用、靶点信息以及任何特殊要求。
让我们成为您最可靠的研发伙伴!
立即联系我们,获取免费的技术咨询与个性化报价!
Professional Antibody and Protein Labeling Services – Illuminating Your Scientific Discoveries
Service Overview
We provide one-stop, high-quality antibody and protein labeling technical services. Utilizing advanced labeling technologies and a strict quality control system, we precisely conjugate various functional labels (such as fluorescent dyes, enzymes, biotin, isotopes, etc.) to your antibodies or proteins, thereby endowing them with "visible" or "detectable" properties. Our services are designed to help you obtain more sensitive, accurate, and reliable data in your research in fields such as immunology, cell biology, drug development, and in vitro diagnostics.
Our Core Labeling Technology Platforms
We possess diverse labeling technologies to meet the needs of different applications:
1. Fluorescent Labeling
o Labels: FITC, TRITC, Cy3, Cy5, Cy7, AF series (e.g., 488, 555, 647), APC, PE, and near-infrared dyes, etc.
o Advantages: High fluorescence brightness, excellent photostability, suitable for multiplex detection.
o Technical Highlights: We optimize reaction conditions to ensure high labeling efficiency and minimal impact on protein activity.
2. Enzyme Labeling
o Labels: Horseradish Peroxidase, Alkaline Phosphatase, β-Galactosidase, etc.
o Advantages: Strong signal amplification, extremely high sensitivity, ideal for ELISA, Western Blot, and Immunohistochemistry.
o Technical Highlights: Precise control of the enzyme-to-antibody ratio ensures high catalytic activity and low background.
3. Biotin Labeling
o Label: Biotin.
o Advantages: Enables multi-stage signal amplification via streptavidin, greatly enhancing detection sensitivity. The biotin-avidin system is one of the most powerful detection systems available.
o Technical Highlights: Controlled biotinylation level to avoid steric hindrance caused by over-labeling.
4. Other Specialized Labeling
o Labels: Small molecule drugs, DNA barcodes, nanoparticles, metal tags, etc.
o Applications: Meets the needs of cutting-edge fields such as ADC drug development, multiplex detection, and mass cytometry.
Our Service Process
We are committed to providing a simple, efficient, and traceable service experience:
1. Project Consultation & Protocol Design: You provide target protein information and experimental objectives; our expert team recommends the most suitable labeling protocol and label.
2. Sample Submission: You provide purified antibody or protein samples (along with information on concentration, buffer composition, etc.).
3. Labeling & Purification:
o Activation & Conjugation: Specific conjugation using optimized chemical methods.
o Purification: Thorough removal of unconjugated free labels using methods such as gel filtration chromatography or dialysis.
4. Quality Control & Analysis:
o Labeling Efficiency Assessment: Calculation of the label-to-protein ratio using UV-Vis spectrophotometry.
o Purity Analysis: Verification of product purity via SDS-PAGE or HPLC.
o Activity Validation: Functional assays like ELISA or Flow Cytometry to verify the biological activity of the labeled protein (Optional).
5. Product Delivery: Final delivery of the labeled product, complete quality control report, and product usage instructions.
Why Choose Our Service?
· Proven Expertise: A team with years of experience, having successfully delivered thousands of labeling projects involving various rare and small-quantity proteins.
· Flexibility & Customization: We not only offer standard labeling services but also excel at providing fully customized labeling solutions tailored to your specific needs.
· Rigorous Quality Control: Every batch of product undergoes strict quality testing, accompanied by a detailed QC report, ensuring reliable and reproducible results.
· High Activity Retention: Our optimized labeling process maximizes the retention of the original affinity and biological activity of your antibody/protein.
· Fast Turnaround Time: Standard services are typically completed within 1-2 weeks, accelerating your research progress.
· Confidentiality & Security: We maintain strict confidentiality regarding customer-provided samples and project information.
Product Application Areas
Our labeled products are widely used in:
· Flow Cytometry: Multiplex analysis of cell surface and intracellular antigens.
· Immunofluorescence & Immunohistochemistry: Localization and quantification of target proteins in tissues and cells.
· ELISA / Chemiluminescence: Highly sensitive protein quantitative detection.
· Western Blot: Qualitative and semi-quantitative analysis of protein expression.
· Protein Microarrays & Biosensing: High-throughput protein interaction studies.
· Drug Target Discovery & ADC Development: Preliminary research for Antibody-Drug Conjugates.
Notes for Clients
· Sample Requirements: Please provide antibody/protein samples with high purity (>90%), high concentration, and in a clean buffer (avoiding substances containing primary amines such as Tris, glycine, etc.).
· Information Provision: Please inform us of your experimental application, target information, and any special requirements whenever possible.
Let us become your most reliable R&D partner!