计算溶液所需的质量、体积或浓度。
概述
液相芯片（Bio-Plex/Luminex）多因子联检实验服务——解锁高通量、多靶标检测新维度
液相芯片多因子联检实验服务，是基于Luminex xMAP® 技术平台的高端检测解决方案。该技术巧妙地融合了流式细胞术、激光检测、数字信号处理以及微球编码技术，能够在一份微量的样本中（低至25-50μL），同时精准地检测多达50-500种不同的目标分子。
我们提供的是一站式专业服务，从实验设计、样本检测到数据分析，助您轻松实现高效、精准的多指标并行研究。
二、 核心技术原理
服务基于三大核心技术模块：
1. 编码微球：
o 核心是直径约5.6微米的聚苯乙烯微球，内部染有不同比例的红、红外两种荧光染料。
o 通过不同比例的搭配，每个微球都拥有独特的“色彩身份证”，从而实现对上百种不同检测项目的精准区分。
2. 探针偶联：
o 我们将针对不同检测目标的捕获抗体（或核酸探针、受体等）分别偶联到不同编码的微球上。每一种编码的微球对应一种待测物。
3. 三重激光检测：
o 分类激光（红色激光，635nm）： 识别微球的“色彩身份证”，确定检测的是哪个指标。
o 报告激光（绿色激光，532nm）： 检测结合在微球上的报告分子（如生物素-链霉亲和素-藻红蛋白）发出的荧光信号强度，该强度与目标分子的浓度成正比。
o 侧向角激光： 监控微球数量，确保检测的统计学有效性。
工作流程简图：
混合编码微球 → 与样本孵育 → 加入生物素化检测抗体 → 加入荧光报告分子 → 上机检测 → 数据分析
三、 核心产品优势
优势 | 与传统方法（如ELISA）对比 |
高通量与高效率 | 一次检测，百项结果。节省宝贵样本、试剂和时间，实验周期缩短数倍。 |
卓越的灵敏度与动态范围 | 检测下限更低（pg/mL级别），动态范围广（3-5个数量级），无需多次稀释样本。 |
节省珍贵样本 | 仅需微量样本即可完成数十上百种因子的检测，尤其适用于穿刺组织液、干细胞培养上清、儿科样本等稀缺样本。 |
数据质量高，重复性好 | 每个指标均基于数百个微球的平均值，统计学意义更强，批内与批间差异小。 |
灵活性与可定制性 | 可根据您的需求，从成熟的预置Panel（如人细胞因子Panel、小鼠磷酸化信号通路Panel）中挑选，或提供定制化服务，自由组合目标指标。 |
四、 主要服务内容
1. 检测项目类型：
o 蛋白检测： 细胞因子、趋化因子、生长因子、磷酸化蛋白、激酶、内分泌激素、自身抗体等。
o 核酸检测： SNP分型、基因表达分析、病原体多重检测等。
2. 服务流程：
o 前期咨询与方案设计： 我们的专家将与您沟通，根据您的研究目标推荐最合适的检测Panel或设计定制方案。
o 样本准备指导： 提供详细的样本采集、处理、保存和运输指南。
o 专业实验操作： 由经验丰富的技术人员使用Bio-Plex 200系统上进行检测。
o 严谨质控： 每批次实验均包含标准曲线和质控样本，确保数据准确可靠。
o 数据分析与报告： 提供原始数据、标准曲线图、浓度计算结果及专业的统计分析报告。
五、 典型应用领域
· 免疫学研究： Th1/Th2/Th17细胞因子谱分析，炎症机制研究。
· 疾病生物标志物开发： 寻找疾病诊断、预后或疗效监控的血清/血浆分子标志物组合。
· 药物研发与药效评估： 在临床前和临床阶段，多维度评估药物对信号通路及免疫系统的影响。
· 癌症研究： 肿瘤微环境中的细胞因子网络、血管生成因子、肿瘤标志物检测。
· 传染病研究： 宿主对病原体感染的免疫应答谱分析。
· 干细胞与再生医学： 评估干细胞分化过程中的分泌组变化。
六、 客户须知
· 样本类型： 血清、血浆、细胞培养上清、组织裂解液、尿液、脑脊液等。
· 样本量要求： 通常建议每个检测孔提供≥50μL的样本体积（具体取决于检测指标数量）。
· 交付周期： 通常在收到样本后的10个工作日 左右交付完整数据报告。
· 保密协议： 我们郑重承诺，对所有客户项目信息及数据严格保密。
七、 为什么选择我们？
· 领先的技术平台： 依托国际公认的Luminex技术，保证数据的权威性和可发表性。
· 丰富的项目经验： 我们已为数百个科研及工业项目提供支持，涵盖多个热门研究领域。
· 专业的技术团队： 从售前咨询到售后分析，全程有资深科学家提供支持。
· 完善的质量体系： 严格的SOP和质控流程，确保每一次结果的可靠与精确。
立即联系我们，获取免费的技术咨询与个性化方案报价！
让我们用强大的多因子联检技术，为您的科研发现注入全新动力！
Liquid Chip (Bio-Plex/Luminex) Multiplex Assay Service – Unlock New Dimensions in High-Throughput, Multi-Target Detection
The Liquid Chip Multiplex Assay Service is a high-end detection solution based on the Luminex xMAP® technology platform. This technology ingeniously integrates flow cytometry, laser detection, digital signal processing, and microsphere encoding, enabling the simultaneous and precise quantification of up to 50-500 different target molecules from a single, minimal sample volume (as low as 25-50μL).
We provide a comprehensive professional service, from experimental design and sample testing to data analysis, helping you effortlessly achieve efficient and accurate parallel multi-analyte research.
II. Core Technology Principles
The service is based on three core technological modules:
1. Encoded Microspheres:
o The core consists of polystyrene microspheres approximately 5.6 microns in diameter, internally stained with different ratios of red and infrared fluorescent dyes.
o Through varying these ratios, each microsphere carries a unique "color-coded ID," allowing precise distinction between hundreds of different detection targets.
2. Probe Conjugation:
o Capture antibodies (or nucleic acid probes, receptors, etc.) specific to different detection targets are covalently coupled to distinct sets of encoded microspheres. Each microsphere set corresponds to a specific analyte.
3. Triple-Laser Detection:
o Classification Laser (Red laser, 635nm): Identifies the microsphere's "color-coded ID," determining which specific analyte is being measured.
o Reporter Laser (Green laser, 532nm): Detects the fluorescence signal intensity from reporter molecules (e.g., Biotin-Streptavidin-Phycoerythrin) bound to the microspheres. This intensity is proportional to the concentration of the target molecule.
o Side-scatter Laser: Monitors microsphere count to ensure the statistical validity of the detection.
Simplified Workflow:
Mix encoded microspheres → Incubate with sample → Add biotinylated detection antibodies → Add fluorescent reporter molecule → Run on analyzer → Data analysis
III. Core Product Advantages
Advantage | Comparison with Traditional Methods (e.g., ELISA) |
High-Throughput & Efficiency | One test, hundreds of results. Saves precious sample, reagents, and time. Reduces experimental turnaround time significantly. |
Superior Sensitivity & Dynamic Range | Lower detection limits (pg/mL level), wider dynamic range (3-5 log), often eliminating the need for multiple sample dilutions. |
Saves Precious Samples | Requires only a minimal sample volume to complete dozens or hundreds of analyte detections, ideal for scarce samples like biopsy tissue fluid, stem cell culture supernatant, pediatric samples, etc. |
High Data Quality & Reproducibility | Each result is based on the median of hundreds of microspheres, providing stronger statistical significance and low intra- and inter-assay variance. |
Flexibility & Customizability | Choose from established pre-configured Panels (e.g., Human Cytokine Panel, Mouse Phosphorylation Signaling Panel) or utilize our custom service to freely combine your target analytes. |
IV. Main Service Offerings
1. Detection Types:
o Protein Detection: Cytokines, Chemokines, Growth Factors, Phosphoproteins, Kinases, Endocrine Hormones, Autoantibodies, etc.
o Nucleic Acid Detection: SNP Genotyping, Gene Expression Analysis, Pathogen Multiplex Detection, etc.
2. Service Process:
o Initial Consultation & Protocol Design: Our experts will consult with you to recommend the most suitable detection panel or design a custom solution based on your research objectives.
o Sample Preparation Guidance: Provide detailed guidelines for sample collection, processing, storage, and shipping.
o Professional Laboratory Operations: Testing is performed by experienced technicians using the Bio-Plex 200 system.
o Rigorous Quality Control: Each batch includes standard curves and quality control samples to ensure data accuracy and reliability.
o Data Analysis & Reporting: We provide raw data, standard curve graphs, calculated concentration results, and professional statistical analysis reports.
V. Typical Application Areas
· Immunology Research: Th1/Th2/Th17 cytokine profiling, inflammatory mechanism studies.
· Disease Biomarker Discovery: Identifying serum/plasma molecular biomarker panels for disease diagnosis, prognosis, or therapeutic monitoring.
· Drug Discovery & Efficacy Evaluation: Multi-dimensional assessment of drug effects on signaling pathways and the immune system in pre-clinical and clinical stages.
· Cancer Research: Detection of cytokine networks, angiogenesis factors, and tumor markers in the tumor microenvironment.
· Infectious Disease Research: Analysis of host immune response profiles to pathogen infection.
· Stem Cell & Regenerative Medicine: Assessing secretome changes during stem cell differentiation.
VI. Client Information
· Sample Types: Serum, Plasma, Cell Culture Supernatant, Tissue Lysates, Urine, Cerebrospinal Fluid, etc.
· Sample Volume Requirements: Typically, ≥50μL per well is recommended (specific volume depends on the number of analytes).
· Turnaround Time: A complete data report is typically delivered within 10 business days upon sample receipt.
· Confidentiality Agreement: We strictly adhere to confidentiality protocols for all client project information and data.
VII. Why Choose Us?
· Leading Technology Platform: Reliance on the internationally recognized Luminex technology ensures authoritative, publication-ready data.
· Extensive Project Experience: We have supported hundreds of research and industrial projects across multiple hot research fields.
· Professional Technical Team: Support from senior scientists is available throughout the entire process, from pre-sales consultation to post-analysis.
· Comprehensive Quality System: Strict SOPs and QC processes ensure reliable and precise results every time.
Contact us now for free technical consultation and a personalized service quote!
Let our powerful multiplex assay technology unlock new dimensions for your research discoveries!
规格
别名
液相芯片, Bio-Plex，Luminex，多因子联检
英文别名
Liquid Chip， Bio-Plex，Luminex，Multiplex Assay
英文名称
Liquid Chip-based Multiplex Assay Services
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