BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
粪便的化学检查有酸碱度反应、隐血试验、胆色素和脂肪测定等,其中隐血试验最具有重要的临床应用价值,上消化道出血量小于5ml,粪便中无可见的血液,且红细胞破坏,显微镜检查也未见红细胞,而需要用化学法、免疫法、转铁蛋白法、血红蛋白荧光测定法等才能证实的出血,称为隐血,检查粪便隐血的试验称为粪便隐血试验(fecal occult blood test,FOBT),化学法常用试剂有邻联甲苯胺、匹拉米洞、愈创木脂、无色孔雀绿、米土尔等。
粪便隐血定性检测试剂盒(邻联甲苯胺法)的检测原理是血红蛋白中的亚铁血红素具有类似过氧化物酶的活性,能催化H2O2作为电子受体使色原底物邻联甲苯胺氧化成邻甲偶氮苯而显绿色→蓝色,其颜色深浅与血红蛋白含量成正比,根据显色的快慢可以半定量血红蛋白即隐血含量,因此也可称作粪便隐血半定量试验,多用于人或动物粪便血红蛋白检查,应注意的是正常人或动物消化道也会微量出血或受到刺激后出血或模型动物饲料含有鱼粉、铁剂等,都可能导致试验结果出现假阳性,本法特点是灵敏度高、假阳性高,可检出Hb 0.2~1.0μg/ml,可联合使用中灵敏度、中特异性的匹拉米洞法。该试剂盒仅用于科研领域,不适用于临床诊断或其他用途。
产品组分表：
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自备材料:
1、粪便标本、木片或牙签、消毒棉或白瓷板、滴管或移液器
操作步骤(仅供参考):
1、粪便标本应尽快收集并及时检测,避免长时间放置使反应的灵敏度降低,避免接触水。
2、用取样木片或牙签挑取少量粪便10~50mg(绿豆大小),涂在消毒棉或白瓷板上。
3、滴加O-tolidine Solution 2滴(约0.1ml)于粪便不同位置。
4、滴加氧化剂2滴(约0.1ml),立即计时并观察颜色变化。
5、于2min内判读完毕,如2min内有蓝色出现,提示粪便含有血红蛋白(Hb)或粪便隐血试验阳性。
2、试剂开封后请尽快使用,以防影响后续实验效果。
结果判读:
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注意事项:
1、在粪便标本采集、运送过程中和使用试剂进行检测时,操作人员应做好个人防护,避免人体接触,以防止可能产生的污染和自身感染。
2、正常人或动物消化道也会微量出血或受到刺激后出血,导致本法有时会出现假阳性。
3、对无任何明显症状但怀疑可能有少量出现的情况,建议至少连续3天取样,并从不同样本部位取材做两次实验。
4、应提前3天以上禁止食用动物血、肉、肝脏及富含叶绿素食物、铁剂、中药或其他特殊药物,以免出现假阳性反应。
5、对于结肠炎模型的实验动物,饲料成分不应含有鱼粉、铁剂或其他特殊药物,以免出现假阳性反应。
6、O-tolidine Solution 含有乙酸,刺激性比较大,应在通风橱里操作。
7、氧化剂应注意密封4℃保存,6个月之后效率可能会下降;可考虑市售过氧化氢(30%)或过氧化脲配置1~3%的溶液。
8、如有必要可用血液或血红蛋白溶液做梯度对照实验。
9、该试剂盒不能与氧化剂共同存放,并避免阳光直射及高低温储存环境。
有效期: 12个月有效;低温运输,4℃保存。
附表:
表1 粪便隐血试验化学法的干扰因素与评价
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表2 粪便隐血试验化学法的方法学评价
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不同浓度血红蛋白的显色效果对比不同浓度血红蛋白的显色效果对比
(从左至右血红蛋白溶液浓度依次为10μg/ml、50μg/ml、200μg/ml)
物理外观：Liquid
是否无菌：否
Chemical examination of feces includes tests for pH reaction, occult blood, bile pigments, and fat determination, among which the occult blood test holds the most significant clinical value. When upper gastrointestinal bleeding is less than 5 mL, there is no visible blood in the feces, and red blood cells are destroyed and cannot be detected by microscopy, such bleeding is termed "occult." Tests to detect this blood in feces are called fecal occult blood tests (FOBT). Chemical methods commonly use reagents such as o-tolidine, pyramidon, guaiac, colorless malachite green, and Metol.
The detection principle of this Fecal Occult Blood Qualitative Detection Kit (o-Tolidine Method) is based on the fact that heme in hemoglobin possesses peroxidase-like activity. It catalyzes H₂O₂ as an electron acceptor to oxidize the chromogenic substrate o-tolidine into o-tolidine blue, producing a color change from green to blue. The intensity of the color is proportional to the hemoglobin concentration. Based on the speed of color development, the hemoglobin (i.e., occult blood) content can be semi-quantified. Therefore, this test can also be referred to as a semi-quantitative fecal occult blood test. It is primarily used for examining human or animal fecal hemoglobin. It should be noted that trace bleeding from the normal human or animal digestive tract, bleeding caused by irritation, or animal feed containing fish meal, iron supplements, etc., may lead to false-positive results. This method is characterized by high sensitivity and a high false-positive rate, capable of detecting Hb at 0.2–1.0 μg/mL. It can be used in conjunction with the pyramidon method, which offers moderate sensitivity and specificity.
This product is intended for research use only and is not suitable for clinical diagnosis or other purposes.
| D1354183 | Component | 100 T | 300 T | Storage |
| D1354183A | O-Tolidine Solution | 10 ml | 30 ml | 2-8℃. Store in the dark. |
| D1354183B | Oxidizing Agent | 10 ml | 30 ml | 2-8℃ |
Materials to Be Prepared by User
Protocol (For Reference Only)
Fecal specimens should be collected and tested promptly to avoid decreased sensitivity due to prolonged storage. Avoid contact with water.
Use a wooden stick or toothpick to collect a small amount of feces (10–50 mg, approximately the size of a mung bean). Smear it onto a cotton swab or white porcelain plate.
Add 2 drops (approximately 0.1 mL) of O-tolidine Solution onto different areas of the fecal sample.
Add 2 drops (approximately 0.1 mL) of Oxidizing Agent. Immediately start timing and observe the color change.
Complete the interpretation within 2 minutes. If a blue color appears within 2 minutes, it indicates the presence of hemoglobin (Hb) in the feces, i.e., a positive fecal occult blood test.
Please use the reagent promptly after opening to prevent affecting subsequent experimental results.
Result Interpretation
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Precautions
During specimen collection, transportation, and kit usage, personnel should take appropriate protective measures to avoid human contact and prevent potential contamination and self-infection.
Trace bleeding from the normal human or animal digestive tract or bleeding caused by irritation may sometimes lead to false-positive results with this method.
For asymptomatic individuals where occult bleeding is suspected, it is recommended to collect samples for at least 3 consecutive days and perform two tests on samples taken from different parts of the specimen.
Consumption of animal blood, meat, liver, chlorophyll-rich vegetables, iron supplements, traditional Chinese medicines, or other specific drugs should be stopped for at least 3 days before testing to avoid false-positive reactions.
For experimental animals in colitis models, the feed should not contain fish meal, iron supplements, or other specific drugs to avoid false-positive results.
The O-tolidine Solution contains acetic acid and is highly irritating. Operations should be performed in a fume hood.
The Oxidizing Agent should be stored sealed at 4°C. Its efficiency may decrease after 6 months. Commercially available hydrogen peroxide (30%) or urea peroxide can be used to prepare a 1–3% solution as an alternative.
If necessary, gradient control experiments can be performed using blood or hemoglobin solution.
The kit should not be stored together with the Oxidizing Agent. Avoid direct sunlight, high temperatures, and freezing.
Appendix Tables
Table 1: Interference Factors and Evaluation for Chemical FOBT Methods
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Table 2: Methodological Evaluation of Chemical FOBT Methods
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Color Development Comparison with Different Hemoglobin Concentrations
Comparison of color development with different hemoglobin concentrations (from left to right: 10 μg/mL, 50 μg/mL, 200 μg/mL)
| D1354183 | Component | 100 T | 300 T | Storage |
| D1354183A | 邻联甲苯胺溶液 | 10 ml | 30 ml | 2-8℃. Store in the dark. |
| D1354183B | 氧化剂 | 10 ml | 30 ml | 2-8℃ |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0636854 |
分析证书 | D1354183 |
| ZJ26F0636855 |
分析证书 | D1354183 |
| ZJ25F0724833 |
分析证书 | D1354183 |
| ZJ26F0332740 |
分析证书 | D1354183 |
| ZJ25F0825727 |
分析证书 | D1354183 |
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